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首页> 外文期刊>Journal of Clinical Microbiology >DNA Macroarray for Identification and Typing of Staphylococcus aureus Isolates
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DNA Macroarray for Identification and Typing of Staphylococcus aureus Isolates

机译：DNA宏阵列用于金黄色葡萄球菌分离物的鉴定和分型

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A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors involved in their expression, (iii) putative virulence proteins and factors controlling their expression, and (iv) proteins produced by mobile elements. The macroarray was hybridized with the cellular DNAs of 80 S. aureus clinical isolates that were previously typed by analyses of their antibiograms and SmaI patterns. The set selected contained unrelated, endemic, and outbreak-related isolates belonging to 45 SmaI genotypes. In a gene content dendrogram, the 80 isolates were distributed into 52 clusters. The outbreak-related isolates were linked in the same or a closely related cluster(s). Clustering based on gene content provided a better discrimination than SmaI pattern analysis for the tested mecA+ isolates that were endemic to Europe. All of the antibiotic resistance genes detected could be correlated with their corresponding phenotypes, except for one isolate which carried a mecA gene without being resistant. The 16 isolates responsible for bone infections were distinguishable from the 12 isolates from uninfected nasal carriers by a significantly higher prevalence of the sdrD gene coding for a putative SD (serine-aspartate) adhesin (in 15 and 7 isolates, respectively). In conclusion, the macroarray designed for this study offers an attractive and rapid typing method which has the advantage of providing additional information concerning the gene content of the isolate of interest.
机译：设计了一个包含465个基因内扩增子的DNA大型阵列，以在物种水平上鉴定金黄色葡萄球菌并键入 S型。金黄色葡萄球菌。选择的基因包括那些编码（i）S的基因。金黄色葡萄球菌特异性蛋白，（ii）介导抗生素抗性的葡萄球菌和肠球菌蛋白及其表达相关因子，（iii）推定的毒性蛋白及其控制表达因子，以及（iv）移动元件产生的蛋白。宏阵列与80 S的细胞DNA杂交。以前通过对其抗菌素谱图和SmaI模式的分析进行分型的金黄色葡萄球菌临床分离株。所选择的组包含不相关，地方性和暴发相关的分离株，这些分离株属于45个SmaI基因型。在基因含量树状图中，将80个分离株分为52个簇。与爆发相关的分离株被链接在相同或紧密相关的簇中。基于基因含量的聚类分析提供了比SmaI模式分析更好的判别标准，用于测试欧洲特有的 mecA + 菌株。除了一种携带 mecA 基因而不具有抗药性的分离株外，所有检测到的抗生素抗性基因都可以与其相应的表型相关。负责骨骼感染的16个分离株与未感染的鼻腔携带者的12个分离株的区别在于，编码假定的SD（丝氨酸-天冬氨酸）粘附素的 sdrD 基因的患病率明显更高（在15和7个分离株中） ， 分别）。总之，为这项研究设计的大分子阵列提供了一种有吸引力的快速分型方法，其优点是可以提供有关目的分离物基因含量的其他信息。

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