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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Evolutionary History of Tubercle Bacilli Assessed by Study of the Polymorphic Nucleotide within the Nitrate Reductase (narGHJI) Operon Promoter
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Molecular Evolutionary History of Tubercle Bacilli Assessed by Study of the Polymorphic Nucleotide within the Nitrate Reductase (narGHJI) Operon Promoter

机译：通过硝酸还原酶（narGHJI）操纵子启动子内多态核苷酸的研究评估结核杆菌的分子进化历史。

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A well-characterized collection of Mycobacterium tuberculosis complex (MTC) isolates, representing all known subspecies as well as some relevant genotypic families of M. tuberculosis, was analyzed for the newly discovered narGHJI ?215 C-to-T promoter single-nucleotide polymorphism (SNP). This point mutation has been shown in earlier studies to be responsible for the differential nitrate reductase activity of M. tuberculosis versus M. bovis. As previously defined by the presence or the absence of the TbD1 genetic locus, the group included both the “modern” W-Beijing, Haarlem, and Central-Asian1 (CAS1) families as well as the “ancestral” East-African-Indian (EAI) clade. Interestingly, among “modern” M. tuberculosis isolates, those previously classified as Principal Genetic Group 1 (PGG1) organisms by katG463-gyrA95 polymorphism analysis did not present the two-banded narGHJI restriction fragment length polymorphism analysis of PCR products pattern common to the other PGG1 MTC members, including the “ancestral” M. tuberculosis isolates. Instead, they showed a one-banded pattern, aligning them with other evolutionarily recent M. tuberculosis isolates of the PGG2 and PGG3 groups, such as Haarlem, Latin-American and Mediterranean (LAM), and X families. The presence of a nitrate reductase producer phenotype in “Mycobacterium canettii” and some “ancestral” M. tuberculosis isolates, despite a two-band ?215C genotype, argues in favor of an alternate mechanism to explain the differential nitrate reductase activity of certain PGG1 subspecies of the MTC. Overall, these findings may help to establish the precise evolutionary history of important genotype families such as W-Beijing and suggest that the ?215T genotype may have contributed the virulence, spread, and evolutionary success of “modern” M. tuberculosis strains compared to the remaining MTC organisms.
机译：一个表征良好的结核分枝杆菌复合物（MTC）分离物集合，代表所有已知的亚种以及 M的一些相关基因型家族。分析结核中新发现的 narGHJI ？215 C-T启动子单核苷酸多态性（SNP）。在较早的研究中已表明这一点突变是造成 M的硝酸盐还原酶活性差异的原因。结核病与 M。牛羊。根据之前是否存在TbD1基因位点来定义，该小组既包括“现代” W-北京，哈勒姆和中亚1（CAS1）家族，也包括“祖先”东非-印度（ EAI）进化枝。有趣的是，在“现代” M中。结核菌分离株，以前被 katG 463 -gyrA归类为主要遗传组1（PGG1）生物。 em> 95 多态性分析未显示其他PGG1 MTC成员共有的PCR产物模式的两带式 narGHJI 限制性片段长度多态性分析，包括“祖先” M。结核病分离株。取而代之的是，它们显示了一个单带模式，使它们与其他进化上最近的 M保持一致。 PGG2和PGG3组的结核病分离株，例如哈莱姆，拉丁美洲和地中海（LAM）和X族。 “ canettii canettii” 和一些“祖先” M中存在硝酸盐还原酶产生型。结核病分离株尽管有两个带状的215C基因型，但仍主张采用另一种机制来解释MTC某些PGG1亚种的硝酸盐还原酶活性的差异。总体而言，这些发现可能有助于建立重要基因型家族（如W-Beijing）的精确进化史，并暗示215T基因型可能有助于“现代” M的毒力，传播和进化成功。与其他MTC生物相比。

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