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首页> 外文期刊>Journal of Clinical Microbiology >DNA Macrorestriction Analysis of Nontypeable Group B Streptococcal Isolates: Clonal Evolution of Nontypeable and Type V Isolates
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DNA Macrorestriction Analysis of Nontypeable Group B Streptococcal Isolates: Clonal Evolution of Nontypeable and Type V Isolates

机译：无法分型的B组链球菌分离株的DNA宏限制分析：无法分型和V型分离株的克隆进化

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Group B streptococci (GBS) are serotyped according to capsular polysaccharide (CPS) type (Ia to VIII); an isolate is classified as nontypeable (NT) if no detectable CPS is found. Surface-localized protein antigens (α, β, R1, and R4) serve as additional markers to classify GBS isolates, which is particularly useful since NT isolates often express one or more of these proteins. To compare genetic resemblance among isolates with similar protein profiles, we studied 58 NT isolates digested with the SmaI macrorestriction enzyme prior to pulsed-field gel electrophoresis (PFGE). Of these 58, 15.5% expressed α only, 20.7% expressed α+β, 15.5% expressed R4, and 25.8% expressed R1,R4, while 22.4% of the isolates expressed no detectable proteins. The largest PFGE profile group, with 48% of the isolates, was group 4, composed primarily of isolates that expressed R1,R4 or no proteins. The second most common profiles were 3 and 32, each with 13.8% of the isolates. Since NT isolates in profile group 4 were highly related to type V isolates, as demonstrated by PFGE profiles, we investigated 45 type V isolates. Two-thirds of the type V isolates within profile group 4 were classified into subgroup 4a, compared to 28.2% of 39 NT isolates. Only 11% of the V/R1,R4 isolates were identical to the prototype group 4 profile, in contrast to 75% of the NT/R1,R4 isolates. A shift of type V isolates into profile 4 subgroups may be indicative of a genetic change over time. PFGE is a valuable approach for comparison of GBS isolate relatedness and for monitoring of NT and typeable GBS isolates for potential clonal divergence.
机译：根据荚膜多糖（CPS）类型（Ia至VIII）对B组链球菌（GBS）进行血清分型;如果未发现可检测的CPS，则将隔离株分类为不可分型（NT）。表面定位的蛋白抗原（α，β，R1和R4）用作GBS分离物分类的附加标记，这特别有用，因为NT分离物通常表达一种或多种这些蛋白。为了比较具有相似蛋白质谱的分离株之间的遗传相似性，我们研究了在脉冲场凝胶电泳（PFGE）之前用SmaI宏限制性酶消化的58种NT分离株。在这58个中，仅15.5％表示α，20.7％表示α+β，15.5％表示R4和25.8％表示R1，R4，而22.4％的分离株中没有检测到蛋白质。最大的PFGE图谱组（占48％的分离株）为第4组，主要由表达R1，R4或无蛋白的分离株组成。第二个最常见的特征是3和32，每个具有13.8％的分离株。由于PFGE图谱证明，第4组概况中的NT分离物与V型分离物高度相关，因此我们研究了45种V型分离物。配置文件组4中的V型分离株的三分之二被分类为亚组4a，而39种NT分离株中的28.2％被分类为亚组4a。与11％的NT / R1，R4分离株相比，只有11％的V / R1，R4分离株与第4组原型相同。 V型分离株向4个亚组转移可能表明随着时间的推移发生了遗传变化。 PFGE是比较GBS分离株相关性和监测NT和可分型GBS分离株潜在克隆差异的有价值的方法。

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