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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Inducible Clindamycin Resistance in Staphylococci by Broth Microdilution Using Erythromycin-Clindamycin Combination Wells
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Detection of Inducible Clindamycin Resistance in Staphylococci by Broth Microdilution Using Erythromycin-Clindamycin Combination Wells

机译:红霉素-克林霉素联合培养液微量稀释法检测葡萄球菌中诱导的克林霉素耐药性

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A study conducted by 11 laboratories investigated the ability of four combinations of erythromycin (ERY) and clindamycin (CC) (ERY and CC at 4 and 0.5, 6 and 1, 8 and 1.5, and 0.5 and 2 μg/ml) in a single well of a broth microdilution panel to predict the presence of inducible CC resistance. Each laboratory tested approximately 30 Staphylococcus aureus isolates and 20 coagulase-negative staphylococcus (CoNS) isolates in a panel using cation-adjusted Mueller-Hinton broth from three different manufacturers. Only the strains resistant to ERY and those susceptible or intermediate to CC were included in the analysis (S. aureus, n = 333; CoNS, n = 97). Results of the D-zone test were used as the gold standard. After an 18-h incubation, the combination of 4 μg/ml ERY and 0.5 μg/ml CC performed the best, with 98 to 100% sensitivity and 100% specificity for both organism groups. After a 24-h incubation, the ERY-CC combinations of 4 and 0.5, 6 and 1, and 8 and 1.5 μg/ml correlated well with the D-zone test.
机译:由11个实验室进行的一项研究调查了红霉素(ERY)和克林霉素(CC)的四种组合(ERY和CC分别为4和0.5、6和1、8和1.5以及0.5和2μg/ ml)的能力微量稀释板的孔中,以预测诱导的CC抗性的存在。每个实验室使用来自三个不同制造商的阳离子调节的Mueller-Hinton肉汤,在面板中测试了大约30种金黄色葡萄球菌分离株和20种凝固酶阴性葡萄球菌(CoNS)分离株。分析中仅包括对ERY具有抗性的菌株以及对CC敏感或中等的菌株(金黄色葡萄球菌 n = 333; CoNS, n = 97)。 D区测试的结果用作金标准。孵育18小时后,4μg/ ml ERY和0.5μg/ ml CC的组合表现最佳,对两个生物体组的敏感性为98%至100%,特异性为100%。孵育24小时后,4和0.5、6和1、8和1.5μg/ ml的ERY-CC组合与D区测试良好相关。

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