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首页> 外文期刊>Journal of Clinical Microbiology >Human Papillomavirus (HPV) Genotyping Using Paired Exfoliated Cervicovaginal Cells and Paraffin-Embedded Tissues To Highlight Difficulties in Attributing HPV Types to Specific Lesions
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Human Papillomavirus (HPV) Genotyping Using Paired Exfoliated Cervicovaginal Cells and Paraffin-Embedded Tissues To Highlight Difficulties in Attributing HPV Types to Specific Lesions

机译：人类乳头瘤病毒（HPV）基因型使用配对脱落的宫颈阴道细胞和石蜡包埋的组织来突出显示将HPV类型归因于特定病变的困难

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Defining type-specific human papillomavirus (HPV) infections within cervical tissues is important for understanding the pathogenesis of cervical neoplasia and assessing the effectiveness of prophylactic vaccines with limited type-specific spectra. We compared HPV DNA-testing results from 146 matched exfoliated-cell and formalin-fixed-tissue specimens collected by cervicovaginal lavage (CVL) within 90 days of each other from women with histologically confirmed cervical intraepithelial lesions (CIN). The CVL specimens were HPV typed using a MY09/11 L1 consensus primer PCR method followed by dot blot hybridization. The tissue specimens were HPV typed using an SPF10 line probe assay HPV detection system. Of the 146 specimen pairs with evidence of CIN in the tissue, 91.8% were positive for one or more HPV types in both the tissue and cellular specimens. Tissue sections were more likely to be HPV negative (P < 0.01). Typing directly from tissue sections resolved multiple infections detected in exfoliated cells to a single HPV type in only 46.9% of cases. Combined use of both specimen types to attribute lesions to HPV type 16 (HPV-16) and/or -18 led to 43.1% attributed to HPV-16 and/or -18 by both specimen types and 19.9% attributed to HPV-16 and/or -18 by one, but not both, specimen types. Unambiguous attribution of cervical lesions to a single, specific HPV type remains a difficult proposition. Use of multiple specimen types or the development of highly sensitive and robust in situ hybridization HPV-testing methods to evaluate the certainty of attribution of lesions to HPV types might provide insights in future efforts, including HPV vaccine trials.
机译：定义宫颈组织内的特定类型的人乳头瘤病毒（HPV）感染对于了解宫颈肿瘤的发病机制和评估有限的特定类型光谱的预防性疫苗的有效性非常重要。我们比较了在组织学上证实为宫颈上皮内病变（CIN）的女性中，在彼此相隔90天之内通过宫颈阴道灌洗（CVL）收集的146个匹配的脱落细胞和福尔马林固定组织标本中的HPV DNA测试结果。使用MY09 / 11 L1共有引物PCR方法对CVL标本进行HPV分型，然后进行斑点印迹杂交。使用SPF 10 线探针测定HPV检测系统对组织标本进行HPV分型。在146个在组织中具有CIN证据的样本对中，组织和细胞样本中一种或多种HPV类型均为91.8％呈阳性。组织切片更有可能是HPV阴性（ P <0.01）。直接从组织切片中键入可将在脱落细胞中检测到的多种感染分解为单一HPV类型，仅占46.9％的病例。两种标本类型的组合使用将病变归因于HPV 16型（HPV-16）和/或-18导致两种标本类型归因于HPV-16和/或-18的占43.1％，归因于HPV-16和/或18的占19.9％。 /或-18乘以一个（但不是两个）样本类型。明确将宫颈病变归因于单一的特定HPV类型仍然是一个难题。多种标本类型的使用或高度敏感且稳健的原位杂交HPV测试方法的开发，以评估病变归因于HPV类型的确定性，可能会为包括HPV疫苗试验在内的未来工作提供见识。

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