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首页> 外文期刊>Journal of Clinical Microbiology >Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates
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Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates

机译:鸟分枝杆菌亚种中gyrA和gyrB基因的多态性。 I,II和III型肺结核

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The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.
机译:一组鸟分枝杆菌亚种的 gyrA gyrB 基因的分析。 I,II和III型的副结核病分离株检测到特定类型的单核苷酸多态性。根据这些结果,我们开发了一种PCR和限制性内切酶分析方法,以区分I型和III型分离株。当细菌生长不足以进行脉冲场凝胶电泳和IS 900 限制性片段长度多态性时,该技术的应用将是表征这些菌株的独特策略。

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