Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms

Alma C. van de Pol; Anton M. van Loon; Tom F. W. Wolfs; Nicolaas J. G. Jansen; Monique Nijhuis; Els Klein Breteler; Rob Schuurman; John W. A. Rossen

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Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms

机译：实时PCR检测呼吸道症状患者样品中呼吸道合胞病毒，流感病毒，副流感病毒和腺病毒的检测率提高

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Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

机译：通过实时PCR，病毒培养和直接免疫荧光检测呼吸道合胞病毒，流感病毒，副流感病毒和腺病毒的住院症状患者的呼吸道样本（ n = 267）。与传统的诊断测试相比，实时PCR将儿童和成人的这些病毒的诊断产率分别从24％提高到43％，从3.5％提高到36％。 展开▼

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《Journal of Clinical Microbiology》 |2007年第7期|共3页

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Alma C. van de Pol; Anton M. van Loon; Tom F. W. Wolfs; Nicolaas J. G. Jansen; Monique Nijhuis; Els Klein Breteler; Rob Schuurman; John W. A. Rossen;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4 [J] . Kate E. Templeton, Sitha A. Scheltinga, Matthias F. C. Beersma, Journal of Clinical Microbiology . 2004,第4期

机译：使用多重实时PCR的快速灵敏方法诊断甲型流感和乙型流感病毒，呼吸道合胞病毒和副流感病毒1、2、3和4的感染

2. Simultaneous detection of influenza A, B, and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested-PCR assay. [J] . Coiras MT, Perez Brena P, Garcia ML, Journal of Medical Virology . 2003,第1期

机译：通过多重逆转录巢式PCR分析法同时检测临床样品中的甲型，乙型和丙型流感病毒，呼吸道合胞病毒和腺病毒。

3. Evaluation of the One-Step Multiplex Real-Time Reverse Transcription-PCR ProFlu-1 Assay for Detection of Influenza A and Influenza B Viruses and Respiratory Syncytial Viruses in Children [J] . Jér?me LeGoff, Rachid Kara, Florence Moulin, Journal of Clinical Microbiology . 2008,第2期

机译：一步多路实时反转录PCR ProFlu-1检测儿童甲型流感和乙型流感病毒及呼吸道合胞病毒的评估

4. A meta-analysis of vaccine effectiveness against bovine herpesvirus, bovine viral diarrhea virus, bovine respiratory syncytial virus, and parainfluenza-type 3 virus in cattle for bovine respiratory di [C] . M.E. Theurer, R.L. Larson, B.J. White Annual Conference of the American^Association^of^Bovine^Practitioners., Conference . 2014

机译：对牛疱疹病毒，牛病毒性腹泻病毒，牛呼吸道和血酸病毒和牛牛呼吸道牛牛牛呼吸道型病毒和Parainfluenza型3病毒的疫苗效果的荟萃分析

5. Development and Application of a Simple Molecular Short RT-PCR Assay for Detection and Differentiation of Influenza A Viruses. [D] . Benrahoma, Ghada Mohamed. 2013

机译：一种简单的分子短RT-PCR检测和鉴别甲型流感病毒的方法的开发和应用。

6. Increased Detection of Respiratory Syncytial Virus Influenza Viruses Parainfluenza Viruses and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms [O] . Alma C. van de Pol, Anton M. van Loon, Tom F. W. Wolfs, 2007

机译：实时PCR检测呼吸道症状患者样品中呼吸道合胞病毒流感病毒副流感病毒和腺病毒的检测率提高

7. Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms▿ [O] . van de Pol, Alma C., van Loon, Anton M., Wolfs, Tom F. W., 2007

机译：实时PCR检测呼吸道症状患者的样品中呼吸道合胞病毒，流感病毒，副流感病毒和腺病毒的检测率更高▿

8. Development of Attenuated Mutants of Respiratory Syncytial Virus and Parainfluenza Viruses as Potential Vaccine Strains [R] . Simpson, R. W. 1979

机译：作为潜在疫苗株的呼吸道合胞病毒和副流感病毒减毒突变体的研制

1. 呼吸道合胞病毒和腺病毒在小儿急性呼吸道感染时的PCR法检测分析 [J] . 薛承岩 ,韩艳欣 . 承德医学院学报 . 1998,第002期

2. 呼吸道合胞病毒、副流感病毒3型毛细支气管炎患儿鼻咽分泌物趋化因子水平的比较 [J] . 董琳 ,陈小芳 ,周晓聪 . 中华实用儿科临床杂志 . 2007,第004期

3. 呼吸道合胞病毒疫苗和副流感病毒3型疫苗联用可能是安全的 [J] . 张薇 . 传染病网络动态 . 2005,第002期

4. 呼吸道合胞病毒和副流感病毒疫苗的研究进展 [J] . 童一民 ,潘欣 . 国际生物制品学杂志 . 2004,第006期

5. 呼吸道合胞病毒和副流感病毒疫苗研制现状WHO疫… [J] . Jame.E . 国外医学：预防．诊断．治疗用生物制品分册 . 1996,第001期

6. 天津地区呼吸道感染住院患儿呼吸道合胞病毒检测分析 [C] . . 第三届全国中西医结合治疗肝病临床经验学术研讨会 . 2016

7. 1.免疫层析法在流感疑似患者床旁检测中的临床应用;2.常见呼吸道病毒的体外细胞培养与鉴定;3.四种抗病毒药物体外抗流感病毒敏感性研究 [A] . 张英 . 2009

1. 呼吸道合胞病毒、腺病毒、副流感病毒和流感病毒检测引物和探针 [P] . 中国专利： CN101812532A . 2010-08-25

2. 一种基于双扩增技术联合检测呼吸道合胞病毒、副流感病毒及腺病毒的试剂盒及其应用 [P] . 中国专利： CN110964853A . 2020-04-07

3. Method for the production of a virus and / or viral proteins other than adenoviruses or adenoviral proteins, use of a human cell, virus or viral protein for use in a vaccine obtainable by means of the aforementioned methods and use, human cell kit to determine the activity of a protease in a sample, use of a viral protein or a virus, a method for concentrating influenza viruses in conditions capable of, at least in part, preserving the virus' ability to infect, and infectious influenza viruses or their derivatives. [P] . 外国专利： BR0015846A . 2002-07-16

机译：制备除腺病毒或腺病毒蛋白以外的病毒和/或病毒蛋白的方法，人细胞，病毒或病毒蛋白在可通过上述方法获得的疫苗中使用的用途和用途，人细胞试剂盒用于确定样品中蛋白酶的活性，病毒蛋白或病毒的使用，在至少能够保留病毒感染能力的条件下浓缩流感病毒的方法以及传染性流感病毒或其衍生物。

4. Composition, processes for preparing influenza viruses, for preparing a gene release vehicle, for preparing defective infectious replicating influenza viruses, for immunizing a patient against a pathogen, for preparing infectious viruses, and for preparing viruses, viruses, cells, vector host cell and use of virus [P] . 外国专利： BR0009580A . 2002-01-02

机译：组成，制备流感病毒，制备基因释放载体，制备有缺陷的传染性复制流感病毒，为患者免疫病原体，制备感染性病毒以及制备病毒，病毒，细胞，载体宿主细胞的方法和用途病毒

5. MULTIPLEX REAL-TIME PCR DETECTION OF INFLUENZA VIRUSES 2009 H1N1, INFLUENZA A AND INFLUENZA B [P] . 外国专利： US2015133324A1 . 2015-05-14

机译：流感病毒2009 H1N1，流感A和流感B的多重实时PCR检测

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Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms

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