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首页> 外文期刊>Journal of Clinical Microbiology >Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid
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Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid

机译：通过直接在脑脊液中进行基因分型来确定2006年与脑膜炎有关的肠病毒

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Enterovirus infections were investigated with special emphasis on performing rapid molecular identification of enterovirus serotypes responsible for aseptic meningitis directly in cerebrospinal fluid (CSF). Enterovirus genotyping was carried out directly with specimens tested for the diagnostic procedure, using two seminested PCR assays designed to amplify the complete and partial gene sequences encoding the VP1 and VP4/VP2 capsid proteins, respectively. The method was used for identifying the enterovirus serotypes involved in meningitis in 45 patients admitted in 2005. Enterovirus genotyping was achieved in 98% of the patients studied, and we obtained evidence of 10 of the most frequent serotypes identified earlier by genotyping of virus isolates. The method was applied for the prospective investigation of 54 patients with meningitis admitted consecutively in 2006. The enterovirus serotypes involved were identified with the cerebrospinal fluid (CSF) of 52 patients (96%) and comprised 13 serotypes within the human enterovirus B species and 1 within the human enterovirus A species. The three most common serotypes were echovirus 13 (E13; 24%), E6 (23%), and coxsackievirus B5 (11.5%), a pattern different from that observed in 2005. Genotyping of virus isolates was also performed in 35 patients in 2006 (meningitis, n = 31; other diseases, n = 4). By comparison, direct genotyping in CSF yielded a more complete pattern of enterovirus serotypes, thereby allowing the detection of rare serotypes: three less common serotypes (CB2, E21, and E27) were not detected by indirect genotyping alone. The study shows the feasibility of prospective enterovirus genotyping within 1 week in a laboratory setting.
机译：对肠道病毒感染进行了调查，并特别着重于直接在脑脊液（CSF）中对负责无菌性脑膜炎的肠道病毒血清型进行快速分子鉴定。肠道病毒的基因分型是直接通过测试用于诊断程序的标本进行的，使用了两个半嵌套式PCR分析法，设计用于分别扩增编码VP1和VP4 / VP2衣壳蛋白的完整和部分基因序列。该方法用于鉴定2005年收治的45例脑膜炎患者中的肠道病毒血清型。在98％的研究患者中实现了肠道病毒基因分型，我们获得了通过病毒分离物基因分型更早鉴定出的10种最常见血清型的证据。该方法用于2006年连续入院的54例脑膜炎患者的前瞻性研究。通过52例患者（96％）的脑脊液（CSF）鉴定了涉及的肠病毒血清型，其中包括人类肠病毒B属13种血清型和1例在人类肠道病毒A物种中。三种最常见的血清型分别是回声病毒13（E13; 24％），E6（23％）和柯萨奇病毒B5（11.5％），与2005年观察到的模式不同。2006年也对35例患者进行了病毒分离株基因分型（脑膜炎， n = 31;其他疾病， n = 4）。相比之下，CSF中的直接基因分型产生了更完整的肠道病毒血清型模式，从而可以检测罕见的血清型：仅通过间接基因分型无法检测到三种较不常见的血清型（CB2，E21和E27）。该研究表明在实验室环境中在1周内进行前瞻性肠病毒基因分型的可行性。

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