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首页> 外文期刊>Journal of Clinical Microbiology >The EmsB Tandemly Repeated Multilocus Microsatellite: a New Tool To Investigate Genetic Diversity of Echinococcus granulosus Sensu Lato
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The EmsB Tandemly Repeated Multilocus Microsatellite: a New Tool To Investigate Genetic Diversity of Echinococcus granulosus Sensu Lato

机译：EmsB串联重复的多基因座微卫星：一种调查细粒棘球Genetic的遗传多样性的新工具。

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Cystic echinococcosis (CE) is a widespread and severe zoonotic disease caused by infection with the larval stage of the eucestode Echinococcus granulosus sensu lato. The polymorphism exhibited by nuclear and mitochondrial markers conventionally used for the genotyping of different parasite species and strains does not reach the level necessary for the identification of genetic variants linked to restricted geographical areas. EmsB is a tandemly repeated multilocus microsatellite that proved its usefulness for the study of genetic polymorphisms within the species E. multilocularis, the causative agent of alveolar echinococcosis. In the present study, EmsB was used to characterize E. granulosus sensu lato samples collected from different host species (sheep, cattle, dromedaries, dogs, and human patients) originating from six different countries (Algeria, Mauritania, Romania, Serbia, Brazil, and the People's Republic of China). The conventional mitochondrial cox1 and nad1 markers identified genotypes G1, G3, G5, G6, and G7, which are clustered into three groups corresponding to the species E. granulosus sensu stricto, E. ortleppi, and E. canadensis. With the same samples, EmsB provided a higher degree of genetic discrimination and identified variations that correlated with the relatively small-scale geographic origins of the samples. In addition, one of the Brazilian single hydatid cysts presented a hybrid genotypic profile that suggested genetic exchanges between E. granulosus sensu stricto and E. ortleppi. In summary, the EmsB microsatellite exhibits an interesting potential for the elaboration of a detailed map of the distribution of genetic variants and therefore for the determination and tracking of the source of CE.
机译：囊性棘球co病（CE）是一种广泛的，严重的人畜共患病，是由于感染了真核生物细小棘球E的幼虫期而引起的。常规用于不同寄生虫物种和菌株基因分型的核和线粒体标记所显示的多态性未达到鉴定与有限地理区域相关的遗传变异所必需的水平。 EmsB是一个串联重复的多基因座微卫星，证明了其对研究 E内遗传多态性的有用性。 multilocularis ，是肺泡棘球co病的病原体。在本研究中，EmsB用于表征 E。来自六个不同国家（阿尔及利亚，毛里塔尼亚，罗马尼亚，塞尔维亚，巴西和中华人民共和国）的不同宿主物种（绵羊，牛，单峰骆驼，狗和人类患者）采集的颗粒状样品。常规的线粒体 cox1 和 nad1 标记可识别基因型G1，G3，G5，G6和G7，这些基因型分为与 E种相对应的三组。粒状严格意义上的， E。 ortleppi 和 E。 canadensis 。对于相同的样品，EmsB提供了更高程度的遗传歧视，并确定了与样品相对较小的地理起源相关的变异。另外，巴西单包虫囊肿之一表现出杂种基因型谱，提示 E之间存在遗传交换。 granulosus sensu stricto和 E。 Ortleppi 。总而言之，EmsB微卫星在拟定遗传变异分布的详细图谱以及因此确定和追踪CE来源方面展现出有趣的潜力。

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