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首页> 外文期刊>Journal of Clinical Microbiology >Vibrio cholerae Classical Biotype Strains Reveal Distinct Signatures in Mexico
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Vibrio cholerae Classical Biotype Strains Reveal Distinct Signatures in Mexico

机译：霍乱弧菌经典生物型菌株在墨西哥揭示了独特的特征

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Vibrio cholerae O1 classical (CL) biotype caused the fifth and sixth pandemics, and probably the earlier cholera pandemics, before the El Tor (ET) biotype initiated the seventh pandemic in Asia in the 1970s by completely displacing the CL biotype. Although the CL biotype was thought to be extinct in Asia and although it had never been reported from Latin America, V. cholerae CL and ET biotypes, including a hybrid ET, were found associated with areas of cholera endemicity in Mexico between 1991 and 1997. In this study, CL biotype strains isolated from areas of cholera endemicity in Mexico between 1983 and 1997 were characterized in terms of major phenotypic and genetic traits and compared with CL biotype strains isolated in Bangladesh between 1962 and 1989. According to sero- and biotyping data, all V. cholerae strains tested had the major phenotypic and genotypic characteristics specific for the CL biotype. Antibiograms revealed the majority of the Bangladeshi strains to be resistant to trimethoprim-sulfamethoxazole, furazolidone, ampicillin, and gentamicin, while the Mexican strains were sensitive to all of these drugs, as well as to ciprofloxacin, erythromycin, and tetracycline. Pulsed-field gel electrophoresis (PFGE) of NotI-digested genomic DNA revealed characteristic banding patterns for all of the CL biotype strains although the Mexican strains differed from the Bangladeshi strains in 1 to 2 DNA bands. The difference was subtle but consistent, as confirmed by the subclustering patterns in the PFGE-based dendrogram, and can serve as a regional signature, suggesting the pre-1991 existence and evolution of the CL biotype strains in the Americas, independent from Asia.
机译：霍乱弧菌O1经典（CL）生物型引起了第五和第六次大流行，可能是较早的霍乱大流行，在1970年代El Tor（ET）生物型通过完全取代CL生物型引发亚洲第七次大流行之前。尽管认为CL生物型在亚洲已经灭绝，尽管从未在拉丁美洲报告过，但在1991年至1997年之间，发现了霍乱弧菌CL和ET生物型，包括杂种ET，与墨西哥霍乱流行地区有关。在这项研究中，根据主要表型和遗传特征，对从1983年至1997年在墨西哥霍乱流行地区分离的CL生物型菌株进行了表征，并将其与1962年至1989年在孟加拉国分离的CL生物型菌株进行了比较。 ，所有测试的霍乱弧菌菌株均具有CL生物型特有的主要表型和基因型特征。抗菌谱显示，孟加拉国的大多数菌株对甲氧苄啶-磺胺甲基异恶唑，呋喃唑酮，氨苄青霉素和庆大霉素具有抗性，而墨西哥菌株对所有这些药物以及环丙沙星，红霉素和四环素均敏感。 NotI消化的基因组DNA的脉冲场凝胶电泳（PFGE）揭示了所有CL生物型菌株的特征性条带模式，尽管墨西哥菌株与孟加拉国菌株在1-2条DNA条带中有所不同。如基于PFGE的树状图的亚簇模式所证实的，差异是细微但一致的，并且可以作为区域性标志，表明1991年前美洲的CL生物型菌株的存在和进化，独立于亚洲。

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