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首页> 外文期刊>Journal of Clinical Microbiology >Global Improvement in Genotyping of Human Papillomavirus DNA: the 2011 HPV LabNet International Proficiency Study
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Global Improvement in Genotyping of Human Papillomavirus DNA: the 2011 HPV LabNet International Proficiency Study

机译：人类乳头瘤病毒DNA基因分型的全球改进：2011 HPV LabNet国际能力研究

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Accurate and internationally comparable human papillomavirus (HPV) DNA genotyping is essential for HPV vaccine research and for HPV surveillance. The HPV Laboratory Network (LabNet) has designed international proficiency studies that can be issued regularly and in a reproducible manner. The 2011 HPV genotyping proficiency panel contained 43 coded samples composed of purified plasmids of 16 HPV types (HPV6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -56, -58, -59, -66, -68a, and -68b) and 3 extraction controls. Tests that detected 50 IU of HPV16 and HPV18 and 500 genome equivalents for the other 14 HPV types in both single and multiple infections were considered proficient. Ninety-six laboratories worldwide submitted 134 data sets. Twenty-five different HPV genotyping assay methods were used, including the Linear Array, line blot/INNO-LiPA, PapilloCheck, and PCR Luminex assays. The major oncogenic HPV types, HPV16 and HPV18, were proficiently detected in 97.0% (113/116) and 87.0% (103/118) of the data sets, respectively. In 2011, 51 data sets (39%) were 100% proficient for the detection of at least one HPV type, and 37 data sets (28%) were proficient for all 16 HPV types; this was an improvement over the panel results from the 2008 and 2010 studies, when <25 data sets (23% and 19% for 2008 and 2010, respectively) were fully proficient. The improvement was also evident for the 54 laboratories that had also participated in the previous proficiency studies. In conclusion, a continuing global proficiency program has documented worldwide improvement in the comparability and reliability of HPV genotyping assay performances.
机译：准确且具有国际可比性的人乳头瘤病毒（HPV）DNA基因分型对于HPV疫苗研究和HPV监测至关重要。 HPV实验室网络（LabNet）设计了可以定期发布且可重复发布的国际水平研究。 2011 HPV基因分型能力小组包含43个编码样品，这些样品由16种HPV类型的纯化质粒组成（HPV6，-11，-16，-18，-31，-33，-35，-39，-45，-51，-52 ，-56，-58，-59，-66，-68a和-68b）和3个提取控件。在一次和多次感染中检测到50 IU的HPV16和HPV18以及500种基因组当量的其他14种HPV类型的测试被认为是熟练的。全世界的96个实验室提交了134个数据集。使用了25种不同的HPV基因分型检测方法，包括线性阵列，线印迹/ INNO-LiPA，PapilloCheck和PCR Luminex检测。分别在97.0％（113/116）和87.0％（103/118）的数据集中可以正确检测到主要的致癌HPV类型HPV16和HPV18。 2011年，至少有一种HPV类型的检出率达到51％（39％），而所有16种HPV类型的检出率均达到37％（28％）。这是对2008年和2010年研究小组结果的改进，当时只有25个数据集（分别为2008年和2010年的23％和19％）是完全熟练的。 54个实验室也参加了以前的能力研究，这种改进也很明显。总之，一项持续的全球熟练度计划已证明HPV基因分型分析性能的可比性和可靠性在全球范围内得到了改善。

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