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首页> 外文期刊>Journal of Clinical Microbiology >Human Herpesvirus 8 Load in Matched Serum and Plasma Samples of Patients with AIDS-Associated Kaposi's Sarcoma
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Human Herpesvirus 8 Load in Matched Serum and Plasma Samples of Patients with AIDS-Associated Kaposi's Sarcoma

机译：艾滋病相关的卡波西氏肉瘤患者的血清和血浆样品中的人类疱疹病毒8负荷

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摘要

Human herpesvirus 8 (HHV-8) (or Kaposi's sarcoma [KS]-associated herpesvirus) is associated with all forms of KS. HHV-8 DNA load in peripheral blood mononuclear cells (PBMCs) of KS patients has been shown to correlate with the clinical stage of the disease. Studies have been done to assess the HHV-8 viral load in different sample types from KS patients and its clinical relevance. This paper describes the design and evaluation of a quantitative real-time (TaqMan) PCR assay for routine diagnosis of HHV-8 infection. The linear dynamic range was 5 to 5 × 106 copies of HHV-8 DNA (r2 > 0.99). The assay is very sensitive, specific, and easily reproducible (less than 2% variability) and can be used for different clinical samples, such as serum, plasma, and PBMCs. The question of which clinical sample, serum or plasma, is preferable for HHV8 DNA testing was addressed, using this newly developed real-time PCR assay. From 85 patients with diagnosed AIDS-KS, matched plasma and serum samples were collected. Of the 85 patients tested, 35 were positive for HHV-8 DNA in both plasma and serum (41%), 8 were positive in serum but not plasma, and 7 had detectable HHV-8 DNA only in plasma. The HHV-8 load was similar in both plasma and serum, and no significant difference was found. However, more inhibition was seen in the plasma samples with the use of a system quality control, seal herpesvirus type 1. Therefore, our results suggest that serum is the preferred material for HHV-8 load testing, since there is less possible hindrance in the amplification than with plasma.
机译：人疱疹病毒8（HHV-8）（或与卡波西氏肉瘤[KS]相关的疱疹病毒）与所有形式的KS相关。 KS患者外周血单核细胞（PBMC）中的HHV-8 DNA负载已显示与该疾病的临床阶段相关。已经进行了研究以评估来自KS患者的不同样品类型中的HHV-8病毒载量及其临床相关性。本文介绍了用于实时诊断HHV-8感染的定量实时（TaqMan）PCR检测方法的设计和评估。线性动态范围是HHV-8 DNA的5至5×10 6 拷贝（ r 2

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