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首页> 外文期刊>Journal of Clinical Microbiology >Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
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Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation

机译：使用全血进行逆转录酶PCR和病毒分离的登革热病毒检测

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Dengue is one of the most important diseases in the tropical and subtropical regions of the world, with an estimated 2.5 billion people being at risk. Detection of dengue virus infections has great importance for the clinical management of patients, surveillance, and clinical trial assessments. Traditionally, blood samples are collected in serum separator tubes, processed for serum, and then taken to the laboratory for analysis. The use of whole blood has the potential advantages of requiring less blood, providing quicker results, and perhaps providing better sensitivity during the acute phase of the disease. We compared the results obtained by reverse transcriptase PCR (RT-PCR) with blood drawn into tubes containing EDTA and those obtained by RT-PCR with blood samples in serum separator tubes from 108 individuals clinically suspected of being infected with dengue virus. We determined that the extraction of RNA from whole blood followed by RT-PCR resulted in a higher detection rate than the use of serum or plasma. Using a selection of these samples, we also found that our ability to detect virus by direct C6/36 cell culture and mosquito inoculation was enhanced by using whole blood but not to the same extent as that seen by the use of RT-PCR.
机译：登革热是世界热带和亚热带地区最重要的疾病之一，估计有25亿人处于危险之中。登革热病毒感染的检测对于患者的临床管理，监测和临床试验评估非常重要。传统上，将血液样本收集在血清分离管中，进行血清处理，然后送到实验室进行分析。全血的使用具有潜在的优势，即需要更少的血液，提供更快的结果以及在疾病的急性期提供更高的敏感性。我们比较了通过逆转录酶PCR（RT-PCR）和抽入含有EDTA的试管中的血液获得的结果，以及通过RT-PCR与108例临床怀疑感染登革热病毒感染者的血清分离管中的血液样品获得的结果。我们确定从全血中提取RNA，然后进行RT-PCR导致检测率高于使用血清或血浆。通过选择这些样本，我们还发现，通过使用全血可以提高通过直接C6 / 36细胞培养和蚊子接种来检测病毒的能力，但程度不如使用RT-PCR所见。

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