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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Single- and Multilocus Sequence Typing and Toxin Gene Profiling for Characterization of Methicillin-Resistant Staphylococcus aureus
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Comparison of Single- and Multilocus Sequence Typing and Toxin Gene Profiling for Characterization of Methicillin-Resistant Staphylococcus aureus

机译：单基因座和多基因座序列分型和毒素基因谱图表征耐甲氧西林金黄色葡萄球菌的比较

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We compared three novel methicillin-resistant Staphylococcus aureus (MRSA) genotyping methods with multilocus sequence typing (MLST) and spa typing to assess their utility for routine strain typing. The new methods were femA and nuc sequence typing and toxin gene profiling (TGP), using a multiplex-PCR-based reverse line blot assay to detect 13 pyrogenic superantigen and exfoliative toxin genes. Forty-two well-characterized MRSA strains, representing 15 MLSTs or 9 clonal clusters (CCs), were genotyped by all methods. Twenty-two spa, nine femA, and seven nuc sequence types were identified. The femA sequence types correlated exactly with CCs; nuc sequences types were less discriminatory but generally correlated well with femA types and CCs. Ten isolates contained none of 13 toxin genes; TGPs of the remainder comprised 1 to 5 toxin genes. The combination of spa typing and TGPs identified 26 genotypes among the 42 strains studied. A combination of two or three rapid, inexpensive genotyping methods could potentially provide rapid MRSA strain typing as well as useful information about clonal origin and virulence.
机译：我们比较了三种新型耐甲氧西林金黄色葡萄球菌（MRSA）基因分型方法与多基因座序列分型（MLST）和 spa 分型，以评估其在常规菌株分型中的效用。新方法是 femA 和 nuc 序列分型和毒素基因概况分析（TGP），使用基于多重PCR的反向线印迹法检测13种热原超抗原和脱落性毒素基因。用所有方法对代表15个MLST或9个克隆簇（CC）的42个特征明确的MRSA菌株进行基因分型。确定了22个 spa ，9个 femA 和7个 nuc 序列类型。 femA 序列类型与CC完全相关; nuc 序列类型的判别性较小，但通常与 femA 类型和CC相关。十个分离物不包含13个毒素基因。其余的TGP包含1至5个毒素基因。通过 spa 分型和TGPs的结合，在所研究的42个菌株中鉴定出26种基因型。两种或三种快速，廉价的基因分型方法的组合可以潜在地提供快速的MRSA菌株分型以及有关克隆起源和毒力的有用信息。

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