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首页> 外文期刊>Journal of Clinical Microbiology >Genomic Characterization of Nontypeable Rotaviruses and Detection of a Rare G8 Strain in Delhi, India
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Genomic Characterization of Nontypeable Rotaviruses and Detection of a Rare G8 Strain in Delhi, India

机译：印度德里的不可分型轮状病毒的基因组表征和罕见的G8菌株的检测

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In the present investigation we molecularly characterized nontypeable rotavirus strains previously identified during surveillance in New Delhi, India. The majority of strains were demonstrated to belong to genotype G1 (54.5%) or P[8] (77.8%) on the basis of nucleotide sequencing of fragments from their VP7 and VP4 genes. The other genotypes detected included G2, G8, G9, G12, and P[4]. A G8P[6] strain, strain DS108, was detected for the first time in northern India. The VP7 gene of DS108 was most homologous with the VP7 gene of a bovine G8 strain, strain A5 (98.9%), indicating its bovine parentage. In contrast, the VP4 gene had a high degree of nucleotide sequence homology (92.9% to 99.1%) with the VP4 genes of human P[6] strains. The VP6 gene and nonstructural genes (NSP1 to NSP3 and NSP5) were most homologous with the VP6 gene and nonstructural genes of human rotaviruses belonging to the DS1 genogroup. Interestingly, the NSP4 gene of DS108 clustered within genotype E6 that until now had only two representative strains, both with G12P[6] specificity (strains RV176-00 and N26-02). Together, these results indicate that G8 strain DS108 belongs to the DS1 genogroup and could be the result of the acquisition of the VP7, VP4, and NSP4 genes by a human G2P[4] strain from more than one donor, similar to the evolution of G12P[6] strain RV176-00. The present study highlights the importance of characterizing multiple genes of nontypeable rotavirus strains to detect novel strains and get a more complete picture of rotavirus evolution.
机译：在本研究中，我们对先前在印度新德里进行监视期间发现的不可分型轮状病毒株进行了分子鉴定。根据来自其VP7和VP4基因的片段的核苷酸测序，大多数菌株被证明属于基因型G1（54.5％）或P [8]（77.8％）。检测到的其他基因型包括G2，G8，G9，G12和P [4]。印度北部首次检测到G8P [6]菌株DS108。 DS108的VP7基因与牛G8株A5株的VP7基因最同源（98.9％），表明其为牛亲本。相反，VP4基因与人P [6]菌株的VP4基因具有高度的核苷酸序列同源性（92.9％至99.1％）。 VP6基因和非结构基因（NSP1至NSP3和NSP5）与属于DS1基因组的人轮状病毒的VP6基因和非结构基因最同源。有趣的是，DS108的NSP4基因聚集在基因型E6内，该基因型迄今仅具有两个代表性菌株，均具有G12P [6]特异性（菌株RV176-00和N26-02）。总之，这些结果表明G8菌株DS108属于DS1基因组，可能是人类G2P [4]菌株从一个以上供体获得VP7，VP4和NSP4基因的结果，类似于G12P [6]株RV176-00。本研究强调了表征不可分型轮状病毒株的多个基因以检测新菌株并获得轮状病毒进化更完整图片的重要性。

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