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首页> 外文期刊>Journal of Clinical Microbiology >Adenovirus Microsatellite Reveals Dynamics of Transmission during a Recent Epidemic of Human Adenovirus Serotype 14 Infection
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Adenovirus Microsatellite Reveals Dynamics of Transmission during a Recent Epidemic of Human Adenovirus Serotype 14 Infection

机译：腺病毒微卫星揭示了人类腺病毒血清型14感染最近流行期间的传播动力学。

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This study reveals diverse-length polymorphisms in long mononucleotide repeats (microsatellites) in several serotypes of epidemic human respiratory adenovirus. The length of one of these microsatellites, a homopolymeric thymidine [poly(T)] repeat, is measured in 68 isolates of adenovirus serotype 14. These isolates were collected during a series of sudden and sometimes fatal outbreaks among both military recruits and civilians as the virus emerged for the first time in the United States in 2006 and 2007. The results demonstrate the usefulness of adenoviral microsatellites as high-resolution molecular strain markers. The described homopolymer is hypervariable in length, varying from 12 to 17 bp in the analyzed sample set. All intermediate lengths were identified in at least one isolate. Furthermore, the specific length of the marker is stable for significant periods of time (up to 7 months) at individual sites where the virus is in consistent circulation. The microsatellite also can maintain specific length identity through site-to-site transmission events, as determined by the analysis of isolates from three advanced training sites that appeared to be subject to pathogen transfer from one of the affected recruit training installations. Public database searches revealed that the polymorphic nature of the microsatellite extends to other species B serotypes, and that other polymorphic microsatellites can be identified readily in a variety of epidemic respiratory adenovirus clades. This study shows that microsatellites are a ubiquitous source of polymorphic markers for human adenoviruses and demonstrates their use through an epidemiological analysis of isolates from a recent North American epidemic.
机译：这项研究揭示了流行性人类呼吸道腺病毒的几种血清型中长单核苷酸重复序列（微卫星）的不同长度多态性。在68株腺病毒血清型14分离株中测量了其中一个微卫星的长度，即均聚胸苷[poly（T）]重复序列。这些分离株是在军事新兵和平民之间一系列突然的，有时是致命的暴发期间收集的。该病毒于2006年和2007年在美国首次出现。结果证明，腺病毒微卫星作为高分辨率分子菌株标记物很有用。所描述的均聚物的长度是高度可变的，在分析的样品组中为12至17 bp。在至少一种分离物中鉴定出所有中间长度。此外，标志物的特定长度在病毒持续流通的各个位点可在相当长的一段时间（最多7个月）内保持稳定。微卫星还可以通过站点到站点的传播事件来保持特定的长度同一性，这是通过分析三个高级培训站点的分离株的分析确定的，这些高级培训站点似乎是从受影响的新兵培训机构之一转移病原体的。公开数据库搜索显示，微卫星的多态性可扩展到其他物种B的血清型，而且在各种流行的呼吸道腺病毒进化枝中也很容易鉴定出其他多态性微卫星。这项研究表明，微卫星是人类腺病毒多态性标记的普遍来源，并通过对近期北美流行病分离株的流行病学分析证明了其用途。

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