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首页> 外文期刊>Journal of Clinical Microbiology >Phylogenetic Analysis of Rubella Viruses Involved in Congenital Rubella Infections in France between 1995 and 2009
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Phylogenetic Analysis of Rubella Viruses Involved in Congenital Rubella Infections in France between 1995 and 2009

机译：1995年至2009年间法国先天性风疹感染涉及的风疹病毒的系统发育分析

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Rubella is an acute infectious disease that normally has a mild clinical course. However, infections during pregnancy, especially before week 12 of gestation (WG), can cause severe birth defects known as congenital rubella syndrome (CRS). The aim of this study was to perform genotyping and molecular characterization of rubella viruses involved in congenital infections in France over the past 15 years (1995 to 2009). Amniotic fluid (AF) specimens (n = 80) from pregnant women with congenital rubella infections (CRI) before week 20 of gestation, and a few other samples available from childrenewborns with CRS (n = 26), were analyzed. The coding region of the rubella virus E1 gene was amplified directly from clinical specimens by reverse transcriptase PCR, and the resulting DNA fragments were sequenced. Sequences were assigned to genotypes by phylogenetic analysis with rubella virus reference sequences. Sufficient E1 gene sequences were obtained from 56 cases. Phylogenetic analysis of the sequences showed that at least five different genotypes (1E, 1G, 1B, 2B, and 1h) were present in France and were involved in congenital infections, with a strong predominance of genotype 1E (87%). This is one of the very few comprehensive studies of rubella viruses involved in CRI. The results indicated that over the past 15 years, multiple introductions of the dominant genotype E caused most of the CRI cases in France. A few sporadic cases were due to other genotypes (1B, 1G, 1h, 2B).
机译：风疹是一种急性感染性疾病，通常具有轻度的临床病程。但是，在怀孕期间，尤其是在妊娠的第12周（WG）之前，感染会导致严重的先天性先天性风疹综合症（CRS）缺陷。这项研究的目的是在过去15年中（1995年至2009年）对法国与先天性感染有关的风疹病毒进行基因分型和分子表征。妊娠第20周前患有先天性风疹感染（CRI）的孕妇的羊水（AF）标本（ n = 80），以及其他一些可从患有CRS的儿童/新生儿获得的其他样品（ n = 26）进行了分析。通过逆转录酶PCR直接从临床标本中扩增风疹病毒E1基因的编码区，并对所得的DNA片段进行测序。通过风疹病毒参考序列的系统发育分析，将序列分配给基因型。从56例病例中获得了足够的E1基因序列。序列的系统发育分析表明，法国存在至少五种不同的基因型（1E，1G，1B，2B和1h），并参与了先天性感染，其中基因型1E的优势很强（87％）。这是参与CRI的风疹病毒极少的全面研究之一。结果表明，在过去的15年中，法国的大多数CRI病例引起了对主要基因型E的多次引入。少数散发病例是由于其他基因型（1B，1G，1h，2B）引起的。

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