Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins

Aufra C. Araujo; Irina V. Astrakhantseva; Howard A. Fields; Saleem Kamili

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Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins

机译：基于对特定HCV结构和非结构蛋白的抗体反应性，区分急性丙型肝炎病毒（HCV）感染

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Currently available serological assays for detection of antibodies to hepatitis C virus (HCV) cannot reliably discriminate acute from chronic HCV infection. We developed a multiplexed, flow-cytometric microsphere immunoassay to measure anti-HCV-IgG reactivities to the core, NS3, NS4, and NS5 HCV recombinant proteins and applied it to 99 serum samples from 24 anti-HCV seroconverters and 141 anti-HCV-IgG and HCV RNA-positive plasma specimens from chronically infected people. Differences in the geometric means or means of signal/cutoff ratios between the two sample sets were statistically significant for all the antigens tested. A multivariate logistic regression model correctly classified the samples in two groups, with a cross-validation accuracy of 90.8% for the acute group and 97.2% for the chronic group. The immunoassay described has the potential to distinguish acute from chronic HCV infection.

机译：当前可用于检测丙型肝炎病毒（HCV）抗体的血清学检测方法不能可靠地将急性与慢性HCV感染区分开。我们开发了一种多重流式细胞术微球免疫测定法，以测量针对核心，NS3，NS4和NS5 HCV重组蛋白的抗HCV-IgG反应性，并将其应用于来自24个抗HCV血清转化器和141个抗HCV-的99个血清样品中来自慢性感染人群的IgG和HCV RNA阳性血浆标本。对于所有测试的抗原，两个样品组之间的几何平均值或信号/截止比平均值的差异在统计学上是显着的。多元逻辑回归模型正确地将样品分为两组，交叉验证的准确率分别为急性组90.8％和慢性组97.2％。所描述的免疫分析方法有可能区分急性和慢性HCV感染。

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《Journal of Clinical Microbiology》 |2011年第1期|共4页
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Aufra C. Araujo; Irina V. Astrakhantseva; Howard A. Fields; Saleem Kamili;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins [J] . Aufra C. Araujo, Irina V. Astrakhantseva, Howard A. Fields, Journal of Clinical Microbiology . 2011,第1期

机译：基于对特定HCV结构和非结构蛋白的抗体反应性，区分急性丙型肝炎病毒（HCV）感染
2. Antigenic structure of the complete nonstructural (NS) 2 and 5 proteins of hepatitis C virus (HCV): anti-HCV NS2 and NS5 antibody reactivities in relation to HCV serotype, presence of HCV RNA, and acute HCV infection. [J] . Z X Zhang, M Chen, A S?nnerborg, Clinical and diagnostic laboratory immunology . 1994,第3期

机译：丙型肝炎病毒（HCV）的完整非结构性（NS）2和5蛋白的抗原结构：与HCV血清型，HCV RNA存在和急性HCV感染有关的抗HCV NS2和NS5抗体反应性。
3. Distinguishing Acute from Chronic and Resolved Hepatitis C Virus (HCV) Infections by Measurement of Anti-HCV Immunoglobulin G Avidity Index [J] . S. Klimashevskaya, A. Obriadina, T. Ulanova, Journal of Clinical Microbiology . 2007,第10期

机译：通过测量抗HCV免疫球蛋白G亲和力指数来区分急性和慢性丙型肝炎病毒（HCV）感染
4. Prevalence of autoantibodies and the risk of autoimmune thyroid disease in children with chronic hepatitis C virus (HCV) infection treated with alpha-interferon [C] . S. GEHRING, E. LAUSCH, U. KULLMER, Falk Symposium . 2002

机译：α-干扰素治疗慢性丙型肝炎病毒（HCV）感染儿童自身抗体和自身免疫性甲状腺疾病风险的患病率
5. Hepatitis C virus (HCV) replication in vitro and the effect of HCV on human immunodeficiency virus (HIV-1) coreceptor usage and diversity in the coinfected patient. [D] . Franco, Kelly Flory. 2007

机译：丙型肝炎病毒（HCV）的体外复制以及HCV对合并感染患者中人免疫缺陷病毒（HIV-1）共受体使用和多样性的影响。
6. Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins [O] . Aufra C. Araujo, Irina V. Astrakhantseva, Howard A. Fields, 2011

机译：基于对特定HCV结构和非结构蛋白的抗体反应性区分急性丙型肝炎病毒（HCV）感染
7. Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins▿ [O] . Araujo, Aufra C., Astrakhantseva, Irina V., Fields, Howard A., 2010

机译：基于对特定HCV结构和非结构蛋白的抗体反应性，区分急性丙型肝炎病毒（HCV）感染▿
8. Morbidity and Mortality Weekly Report, Volume 47, No. RR-19, October 16, 1998.211 Recommendations for Prevention and Control of Hepatitis C Virus (HCV) Infection 211 and HCV-Related Chronic Disease [R] . 1998

机译：发病率和死亡率每周报告，第47卷，第RR-19号，1998年10月16日.211预防和控制丙型肝炎病毒（HCV）感染211和HCV相关慢性病的建议

Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins

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