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首页> 外文期刊>Journal of Clinical Microbiology >Expansion of the Midwestern Focus for Human Granulocytic Anaplasmosis into the Region Surrounding La Crosse, Wisconsin
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Expansion of the Midwestern Focus for Human Granulocytic Anaplasmosis into the Region Surrounding La Crosse, Wisconsin

机译：将人类中性粒细胞无浆膜病的中西部重点扩展到威斯康星州拉克罗斯市周围的地区

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摘要

Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis (HGA), shares the same enzootic life cycle as Borrelia burgdorferi, the causative agent of Lyme disease. Although La Crosse, WI, is a well-recognized Lyme disease focus with an abundance of Ixodes scapularis vector ticks and the first documentation of HGA occurred in patients from northwestern Wisconsin, local transmission of A. phagocytophilum has not to date been documented. In this study, we evaluated DNA extracted from 201 ticks captured locally by a real-time PCR that targeted a unique region within msp2, and 24 samples (12%) yielded positive results. The PCR also detected A. phagocytophilum DNA in blood samples obtained from 53 patients with clinical abnormalities consistent with HGA, and sequencing confirmed that the DNA was recovered from the Ap-ha variant of A. phagocytophilum, associated exclusively with human infection. The findings therefore confirmed that the upper Midwestern focus for HGA endemicity now includes the regions immediately surrounding La Crosse, WI. The results also validated the utility of the real-time msp2 PCR test for confirming acute HGA in the clinical setting.
机译：人类粒细胞性胞浆菌病（HGA）的致病原质吞噬性无花果与莱姆病的致病性伯氏疏螺旋体具有相同的动物生命周期。尽管威斯康星州拉克罗斯（La Crosse）是公认的莱姆病病灶，有大量的肩I突触媒矢量tick，并且最早的HGA文献发生在威斯康星州西北部的患者中，但迄今尚无吞噬嗜血曲霉的局部传播记录。在这项研究中，我们评估了通过实时PCR捕获的本地201 s的DNA提取物，这些DNA靶向了 msp2 中的唯一区域，有24个样本（占12％）产生了阳性结果。 PCR还检测了从53名与HGA一致的临床异常患者中获得的血液样本中的嗜A.phagocytophilum DNA，测序证实该DNA是从嗜A.phagocytophilum的Ap-ha变体中回收的，该变体仅与人类感染有关。因此，研究结果证实，HGA流行的中西部地区重点包括威斯康星州拉克罗斯附近的地区。结果还证实了实时 msp2 PCR检测在临床中确认急性HGA的实用性。

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