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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory-Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens
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Comparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory-Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens

机译：将Simplexa HSV 1和2 PCR与培养，免疫荧光和实验室开发的TaqMan PCR进行比较以检测拭子样本中的单纯疱疹病毒

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The Simplexa HSV 1 & 2 direct PCR assay was compared with conventional cell culture, cytospin-enhanced direct fluorescent antibody (DFA), and a laboratory-developed real-time TaqMan PCR (LDT HSV PCR) using extracted nucleic acid for the detection of herpes simplex virus (HSV) in dermal, genital, mouth, ocular, and other swab samples. One hundred seventy-one swabs were tested prospectively, and 58 were positive for HSV (34 HSV-1 and 24 HSV-2). Cytospin-DFA detected 50 (86.2%), conventional cell culture 51 (87.9%), Simplexa direct 55 (94.8%), and LDT HSV PCR 57 (98.3%) of 58 true positives. Simplexa direct detected more positives than DFA and culture, but the differences were not significant (P = 0.0736 and P = 0.3711, respectively, by the McNemar test). Samples that were positive by all methods (n = 48) were strong positives (LDT cycle threshold [CT] value, 14.4 to 26.1). One strongly positive sample was falsely negative by LDT HSV PCR due to a failure of TaqMan probe binding. Three samples falsely negative by Simplexa direct had high CT values by LDT HSV PCR (LDT CT, 35.8 to 38.2). Omission of the DNA extraction step by Simplexa direct led to a drop in sensitivity compared to the sensitivity of LDT HSV PCR using extracted samples (94.8% versus 98.3%, respectively), but the difference was not significant (P = 0.6171). Simplexa HSV 1 & 2 direct PCR was the most expensive but required the least training of the assays used, had the lowest hands-on time and fastest assay time (75 min, versus 3 h by LDT HSV PCR), and provided the HSV type.
机译：将Simplexa HSV 1和2直接PCR测定法与常规细胞培养，细胞旋转增强型直接荧光抗体（DFA）和实验室开发的实时TaqMan PCR（LDT HSV PCR）进行了比较，使用提取的核酸检测疱疹真皮，生殖器，口腔，眼和其他拭子样本中的单纯形病毒（HSV）。前瞻性测试了171个拭子，其中58例为HSV阳性（34例HSV-1和24例HSV-2）。 Cytospin-DFA检测到了58个阳性样本中的50个（86.2％），常规细胞培养物51个（87.9％），Simplexa direct 55个（94.8％）和LDT HSV PCR 57个（98.3％）。 Simplexa直接检测法比DFA和培养法检测出更多阳性，但差异不显着（通过McNemar检验分别为P = 0.0736和P = 0.3711）。所有方法均为阳性（n = 48）的样品均为强阳性（LDT循环阈值[CT]值为14.4至26.1）。由于TaqMan探针结合失败，LDT HSV PCR检测到一个强阳性样品为假阴性。通过Simplexa Direct假阴性的三个样品通过LDT HSV PCR的CT值较高（LDT CT为35.8至38.2）。与使用提取样品的LDT HSV PCR的灵敏度相比，Simplexa省略了DNA提取步骤直接导致灵敏度下降（分别为94.8％和98.3％），但差异不显着（P = 0.6171）。 Simplexa HSV 1和2直接PCR是最昂贵的方法，但需要的训练最少，其动手时间最短，测定时间最快（75分钟，而LDT HSV PCR为3小时），并且提供了HSV类型。

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