...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >An International, Prospective, Multicenter Evaluation of the Combination of AdvanDx Staphylococcus QuickFISH BC with mecA XpressFISH for Detection of Methicillin-Resistant Staphylococcus aureus Isolates from Positive Blood Cultures
【24h】

An International, Prospective, Multicenter Evaluation of the Combination of AdvanDx Staphylococcus QuickFISH BC with mecA XpressFISH for Detection of Methicillin-Resistant Staphylococcus aureus Isolates from Positive Blood Cultures

机译：对AdvanDx金黄色葡萄球菌QuickFISH BC与mecA XpressFISH的组合进行国际前瞻性多中心评估，以检测阳性血液培养物中耐甲氧西林的金黄色葡萄球菌分离物

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sepsis caused by Staphylococcus aureus is a major health problem worldwide. Better outcomes are achieved when rapid diagnosis and determination of methicillin susceptibility enable early optimization of antimicrobial therapy. Eight large clinical laboratories, seven from the United States and one from Scotland, evaluated the combination of the Staphylococcus QuickFISH BC and the new mecA XpressFISH assay (both AdvanDx, Woburn, MA, USA) for the detection of methicillin-resistant S. aureus in positive blood cultures. Blood cultures flagged as positive by automated blood culture instruments and demonstrating only Gram-positive cocci in clusters on Gram stain were tested by QuickFISH, a 20-min assay. If only S. aureus was detected, mecA XpressFISH testing followed. The recovered S. aureus isolates were tested by cefoxitin disk diffusion as the reference method. The QuickFISH assay results were concordant with the routine phenotypic testing methods of the testing laboratories in 1,211/1,221 (99.1%) samples and detected 488/491 S. aureus organisms (sensitivity, 99.4%; specificity, 99.6%). Approximately 60% of the samples (730) contained coagulase-negative staphylococci or nonstaphylococci as assessed by the QuickFISH assay and were not tested further. The 458 compliant samples positive exclusively for S. aureus by the QuickFISH assay were tested by the mecA XpressFISH assay, which detected 209 of 211 methicillin-resistant S. aureus organisms (sensitivity, 99.1%; specificity, 99.6%). The mecA XpressFISH assay also showed high reproducibility, with 534/540 tests performed by 6 operators over 5 days achieving reproducible results (98.9% agreement). The combination of the Staphylococcus QuickFISH BC and mecA XpressFISH assays is sensitive, specific, and reproducible for the detection of methicillin-resistant S. aureus and yields complete results in 2 h after the blood culture turns positive.
机译：由金黄色葡萄球菌引起的败血症是世界范围内的主要健康问题。快速诊断和确定甲氧西林药敏性可以尽早优化抗菌药物治疗，可获得更好的结果。八个大型临床实验室（来自美国的七个实验室和来自苏格兰的一个实验室）评估了葡萄球菌 Quick FISH BC和新的 mecA Xpress FISH检测的组合（AdvanDx，Woburn都（美国麻萨诸塞州马萨诸塞州），用于检测阳性血液培养物中耐甲氧西林的金黄色葡萄球菌。通过自动血液培养仪标记为阳性且仅在革兰氏染色上呈簇状显示革兰氏阳性球菌的血液培养物通过 Quick FISH（20分钟分析）进行测试。如果仅检测到金黄色葡萄球菌，则进行 mecA Xpress FISH测试。回收的金黄色葡萄球菌分离物通过头孢西丁圆盘扩散法作为参考方法进行测试。 Quick FISH检测结果与测试实验室在1,211 / 1,221（99.1％）个样品中的常规表型测试方法一致，并且检测到488/491个金黄色葡萄球菌生物（敏感性为99.4％;特异性为99.6％）。根据 Quick FISH分析评估，大约60％的样品（730）含有凝固酶阴性葡萄球菌或非葡萄球菌，未经进一步测试。通过 Quick FISH检测法对仅金黄色葡萄球菌呈阳性的458份标本进行了 mecA Xpress FISH检测，检测了211种耐甲氧西林金黄色葡萄球菌中的209种。 （敏感性为99.1％;特异性为99.6％）。 mecA Xpress FISH检测法也显示出高重复性，由6名操作人员在5天之内进行的534/540检测达到了可重复的结果（98.9％的一致性）。金黄色葡萄球菌 Quick FISH BC和 mecA Xpress FISH检测的组合灵敏，特异且可重复，可检测耐甲氧西林的金黄色葡萄球菌，并在2中获得完整结果血液培养阳性后h。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号