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首页> 外文期刊>Journal of Clinical Microbiology >Use of Various Common Isolation Media To Evaluate the New VITEK 2 Colorimetric GN Card for Identification of Burkholderia pseudomallei
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Use of Various Common Isolation Media To Evaluate the New VITEK 2 Colorimetric GN Card for Identification of Burkholderia pseudomallei

机译：使用各种常见的隔离媒体评估新的VITEK 2比色GN卡以鉴定拟假性伯克霍尔德氏菌

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The use of automated systems in the modern microbiology laboratory is becoming commonplace as the pressure of cost containment impacts on staff resources. With increased international travel and threats of bioterrorism, recognition and accurate identification of organisms such as Burkholderia pseudomallei is important. In areas where this organism is endemic, identification is not usually problematic. This study evaluates the performance of the new VITEK 2 colorimetric GN card for the identification of this organism. A total of 103 previously identified clinical isolates were tested with the new card with isolates taken from MacConkey agar, Columbia horse blood agar, Columbia sheep blood agar, and Trypticase soy agar in order to determine identification performance and to see if there was any variability in results due to the agar. Columbia horse blood agar produced the highest rates of identification (81%), followed by Trypticase soy agar (78%), Columbia sheep blood agar (75%), and MacConkey agar (63%). There was considerable variability in some of the reactions obtained. Seven isolates failed to identify from any of the agars used. This study highlights issues with the identification of this organism with the new VITEK 2 GN card. Enhancements of the algorithm parameters for the GN card are warranted and are in progress. Laboratory personnel need to be aware of the current limitations with this GN card and the software (version 4.02 or older for the VITEK 2 60/XL and version 1.02 or older for VITEK 2 Compact) and rely on clinical history, a high index of suspicion, and basic microbiology tests to confirm the identification of this organism.
机译：随着成本控制压力对员工资源的影响，在现代微生物实验室中使用自动化系统正变得司空见惯。随着国际旅行的增加和生物恐怖主义的威胁，识别和准确识别 Burkholderia pseudomallei 等生物非常重要。在这种生物流行的地区，识别通常不会有问题。这项研究评估了新型VITEK 2比色GN卡在鉴定这种生物时的性能。用新卡测试了总共103种先前鉴定出的临床分离株，这些分离株分别来自MacConkey琼脂，Columbia马血琼脂，Columbia羊血琼脂和Trypticase大豆琼脂，以确定其鉴定性能并查看其是否存在变异性。结果由于琼脂。哥伦比亚马血琼脂的鉴定率最高（81％），其次是胰蛋白酶大豆琼脂（78％），哥伦比亚绵羊血琼脂（75％）和麦康凯琼脂（63％）。在获得的某些反应中存在相当大的可变性。七个分离株未能从所用的任何琼脂中鉴定出来。这项研究突出了使用新的VITEK 2 GN卡鉴定该生物的问题。 GN卡的算法参数的增强功能已得到保证并且正在开发中。实验室人员需要了解此GN卡和软件（VITEK 2 60 / XL的版本为4.02或更高版本，VITEK 2 Compact的版本为1.02或更高版本）当前的局限性，并依赖于临床病史，高度怀疑的指标以及基本的微生物学测试，以确认该生物体的身份。

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