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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Epidemiology of Bovine Coronavirus on the Basis of Comparative Analyses of the S Gene
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Molecular Epidemiology of Bovine Coronavirus on the Basis of Comparative Analyses of the S Gene

机译：基于S基因比较分析的牛冠状病毒分子流行病学

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Bovine coronavirus (BCoV), a group 2 member of the genus Coronavirus in the family Coronaviridae, is an important pathogen in cattle worldwide. It causes diarrhea in adult animals (winter dysentery), as well as enteric and respiratory diseases in calves. The annual occurrence of BCoV epidemics in Sweden and Denmark led to this investigation, with the aim to deepen the knowledge of BCoV epidemiology at the molecular level. A total of 43 samples from outbreaks in both countries were used for PCR amplification and direct sequencing of a 624-nucleotide fragment of the BCoV S gene. Sequence comparison and phylogenetic studies were performed. The results showed (i) identical sequences from different animals in the same herds and from paired nasal and fecal samples, suggesting a dominant virus circulating in each herd at a given time; (ii) sequence differences among four outbreaks in different years in the same herd, indicating new introduction of virus; (iii) identical sequences in four different Danish herds in samples obtained within 2 months, implying virus transmission between herds; and (iv) that at least two different virus strains were involved in the outbreaks of BCoV in Denmark during the spring of 2003. This study presents molecular data of BCoV infections that will contribute to an increased understanding of BCoV epidemiology in cattle populations.
机译：牛冠状病毒（BCoV）是冠状病毒科（emonaCoronaviridae）的第2类冠状病毒（emona）的第2组成员，是世界范围内重要的牛病原体。它会引起成年动物的腹泻（冬季痢疾）以及小牛的肠道和呼吸系统疾病。瑞典和丹麦每年发生的BCoV流行病导致了这一调查，目的是在分子水平上加深BCoV流行病学的知识。来自两个国家暴发的总共43个样本用于BCoV S基因的624个核苷酸片段的PCR扩增和直接测序。进行了序列比较和系统发育研究。结果显示：（i）来自同一群中的不同动物以及成对的鼻和粪便样品的相同序列，表明在给定时间在每群中传播有优势病毒; （ii）同一群中不同年份的四次暴发之间的序列差异，表明新引入了病毒; （iii）在两个月内获得的样品中四个不同的丹麦畜群中的序列相同，这意味着病毒在畜群之间传播; （iv）至少有两种不同的病毒株与2003年春季在丹麦的BCoV爆发有关。这项研究提供了BCoV感染的分子数据，这将有助于加深对牛群中BCoV流行病学的了解。

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