Evaluation of a New Rapid Antigen Test Using Immunochromatography for Detection of Human Metapneumovirus in Comparison with Real-Time PCR Assay

Yoko Matsuzaki; Emi Takashita; Michiko Okamoto; Katsumi Mizuta; Tsutomu Itagaki; Fumio Katsushima; Yuriko Katsushima; Yukio Nagai; Hidekazu Nishimura

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Evaluation of a New Rapid Antigen Test Using Immunochromatography for Detection of Human Metapneumovirus in Comparison with Real-Time PCR Assay

机译：与免疫荧光定量PCR相比，使用免疫色谱法检测人偏肺病毒的新型快速抗原检测的评估

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A new rapid human metapneumovirus (hMPV) detection kit using immunochromatography (SAS hMPV test) was compared to real-time PCR for 224 nasal swab specimens, 96.4% of which were obtained from children of <15 years of age. The overall sensitivity and specificity were 82.3% and 93.8%, respectively, suggesting that this test is useful for pediatricians to diagnose hMPV infection in a clinical setting.

机译：将使用免疫色谱法（SAS hMPV测试）的新型快速人间肺炎病毒（hMPV）检测试剂盒与实时PCR进行了比较，比较了224个鼻拭子样本，其中96.4％的样本来自15岁以下的儿童。总体敏感性和特异性分别为82.3％和93.8％，这表明该测试对儿科医生在临床环境中诊断hMPV感染很有用。

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《Journal of Clinical Microbiology》 |2009年第9期|共4页
作者
Yoko Matsuzaki; Emi Takashita; Michiko Okamoto; Katsumi Mizuta; Tsutomu Itagaki; Fumio Katsushima; Yuriko Katsushima; Yukio Nagai; Hidekazu Nishimura;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Comparison of a Lateral-Flow Immunochromatography Assay with Real-Time Reverse Transcription-PCR for Detection of Human Metapneumovirus [J] . Hideaki Kikuta, Chikako Sakata, Reiko Gamo, Journal of Clinical Microbiology . 2008,第3期

机译：侧向流免疫色谱分析与实时逆转录PCR检测人类偏肺病毒的比较
2. Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of Mycoplasma bovis: Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay ‡ [J] . Alesia Reinisch, Daniela Bruno, Debabrata Mahapatra, Veterinary Sciences . 2015,第1期

机译：快速检测牛支原体的新型Taqman实时PCR检测方法的开发和评估：与常规PCR检测方法和另一种Taqman实时PCR检测方法的检测性能比较
3. Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay [J] . P. Jokela, H. Piiparinen, K. Luiro, Clinical Microbiology and Infection . 2010,第10期

机译：与直接荧光法相比，双重实时RT-PCR法检测人间质肺病毒和呼吸道合胞病毒
4. Comparison of ELISA assays and PCR for the detection of anti-PCV2-antlbodies and PCV2-antIgen or DNA in porcine serum, oral fluids, and fecal samples [C] . T. Opriessnig, J. Johnson, D. M. Madson, Annual Meeting of the American Association of Swine Veterinarians . 2007

机译：ELISA测定和PCR检测抗PCV2-砧酵母和PCV2-抗原或DNA在猪血清，口服液和粪便样品中的比较
5. Rapid and sensitive detection of aflatoxin in animal feeds and food grains using immunomagnetic bead based recovery and real-time immuno quantitative PCR (RT-iqPCR) assay. [D] . Babu, Dinesh. 2010

机译：使用基于免疫磁珠的回收率和实时免疫定量PCR（RT-iqPCR）分析快速，灵敏地检测动物饲料和粮食中的黄曲霉毒素。
6. Evaluation of a New Rapid Antigen Test Using Immunochromatography for Detection of Human Metapneumovirus in Comparison with Real-Time PCR Assay [O] . Yoko Matsuzaki, Emi Takashita, Michiko Okamoto, 2009

机译：与实时PCR分析相比使用免疫色谱法检测人偏肺病毒的新型快速抗原测试的评估
7. Evaluation of a New Rapid Antigen Test Using Immunochromatography for Detection of Human Metapneumovirus in Comparison with Real-Time PCR Assay▿ [O] . Matsuzaki, Yoko, Takashita, Emi, Okamoto, Michiko, 2009

机译：与实时PCR分析相比，使用免疫色谱法检测人偏肺病毒的新型快速抗原测试的评价▿

Evaluation of a New Rapid Antigen Test Using Immunochromatography for Detection of Human Metapneumovirus in Comparison with Real-Time PCR Assay

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