Misidentification of Saprochaete clavata as Magnusiomyces capitatus in Clinical Isolates: Utility of Internal Transcribed Spacer Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Importance of Reliable Databases

Marie Desnos-Ollivier; Catherine Blanc; Dea Garcia-Hermoso; Damien Hoinard; Alexandre Alanio; Fran?oise Dromer

首页> 外文期刊>Journal of Clinical Microbiology >Misidentification of Saprochaete clavata as Magnusiomyces capitatus in Clinical Isolates: Utility of Internal Transcribed Spacer Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Importance of Reliable Databases

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Misidentification of Saprochaete clavata as Magnusiomyces capitatus in Clinical Isolates: Utility of Internal Transcribed Spacer Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Importance of Reliable Databases

机译：腐烂的腐烂菌在临床分离株中被鉴定为头孢菌（Magnusiomyces capitatus）：内部转录间隔物测序和基质辅助激光解吸电离的效用–飞行时间质谱法和可靠数据库的重要性

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Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) fingerprints that enable the identification of S. clavata, M. capitatus, and Galactomyces candidus to the species level.

机译：腐烂的矢车菊和人头的巨噬菌是人的病原体，由于它们相似的表型和错误或有限的数据库，经常被彼此误认为。基于内部转录间隔区（ITS）序列，我们提出了物种特异性的碳同化模式和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）指纹图谱，这些指纹图谱可用于识别S. clavata，M。capitatus。和念珠菌（Galactomyces candidus）达到物种水平。

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《Journal of Clinical Microbiology》 |2014年第6期|共3页
作者
Marie Desnos-Ollivier; Catherine Blanc; Dea Garcia-Hermoso; Damien Hoinard; Alexandre Alanio; Fran?oise Dromer;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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机译：通过16S rRNA基因测序，基质辅助激光解吸电离-使用MALDI Biotyper进行飞行时间质谱分析和常规表型分析方法鉴定α-溶血性链球菌临床分离株
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5. Laser desorption/laser ionization time-of-flight mass spectrometry instrument design and investigation of the desorption and ionization mechanisms of matrix-assisted laser desorption/ionization. [D] . King, Richard Charles, III. 1994

机译：激光解吸/激光电离飞行时间质谱仪的设计以及基质辅助激光解吸/电离的解吸和电离机理研究。
6. Misidentification of Saprochaete clavata as Magnusiomyces capitatus in Clinical Isolates: Utility of Internal Transcribed Spacer Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Importance of Reliable Databases [O] . Marie Desnos-Ollivier, Catherine Blanc, Dea Garcia-Hermoso, 2014

机译：腐烂的腐烂菌在临床分离株中被鉴定为头孢菌（Magnusiomyces capitatus）：内部转录间隔物测序和基质辅助激光解吸电离的效用–飞行时间质谱和可靠数据库的重要性
7. Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases [O] . Desnos-Ollivier, Marie, Blanc, Catherine, Garcia-Hermoso, Dea, 2014

机译：在临床分离物中将腐烂的腐烂的腐肉菌鉴定为Capnutus Capitatus：内部转录间隔物测序和基质辅助激光解吸电离飞行时间质谱的实用性以及可靠数据库的重要性

Misidentification of Saprochaete clavata as Magnusiomyces capitatus in Clinical Isolates: Utility of Internal Transcribed Spacer Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Importance of Reliable Databases

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