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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by Puumala Virus
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Molecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by Puumala Virus

机译：肺炎病毒引起的肾综合征出血热的分子诊断

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摘要

Rodent-borne hantaviruses cause two severe acute diseases: hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus pulmonary syndrome (HPS; also called hantavirus cardiopulmonary syndrome [HCPS]) in the Americas. Puumala virus (PUUV) is the most common causative agent of HFRS in Europe. Current routine diagnostic methods are based on serological analyses and can yield inconclusive results. Hantavirus-infected patients are viremic during the early phase of disease; therefore, detection of viral RNA genomes can be a valuable complement to existing serological methods. However, the high genomic sequence diversity of PUUV has hampered the development of molecular diagnostics, and currently no real-time reverse transcription-quantitative (RT)-PCR assay is available for routine diagnosis of HFRS. Here, we present a novel PUUV RT-PCR assay. The assay was validated for routine diagnosis of HFRS on samples collected in Sweden during the winter season from 2013 to 2014. The assay allowed detection of PUUV RNA in 98.7% of confirmed clinical HFRS samples collected within 8 days after symptomatic onset. In summary, this study shows that real-time RT-PCR can be a reliable alternative to serological tests during the early phase of HFRS.
机译：啮齿动物传播的汉坦病毒引起两种严重的急性疾病：在欧亚大陆的肾综合征出血热（HFRS），以及在美洲的汉坦病毒肺综合征（HPS;也称为汉坦病毒心肺综合征[HCPS]）。 Puumala病毒（PUUV）是欧洲最常见的HFRS病原体。当前的常规诊断方法基于血清学分析，可能会产生不确定的结果。汉坦病毒感染的患者在疾病的早期是病毒血症的。因此，病毒RNA基因组的检测可以作为现有血清学方法的宝贵补充。然而，PUUV的高基因组序列多样性阻碍了分子诊断的发展，并且目前尚无用于实时诊断HFRS的实时逆转录定量（RT）-PCR分析方法。在这里，我们提出了一种新颖的PUUV RT-PCR检测方法。该检测方法可用于2013年至2014年冬季在瑞典收集的样本中进行HFRS的常规诊断。该检测方法可在症状发作后8天内，在98.7％的临床HFRS临床样本中检测到PUUV RNA。总而言之，这项研究表明在HFRS早期，实时RT-PCR可以替代血清学检测。

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