...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Comparison of SpectraCell RA Typing and Multilocus Sequence Typing for Extended-Spectrum-β-Lactamase-Producing Escherichia coli
【24h】

Comparison of SpectraCell RA Typing and Multilocus Sequence Typing for Extended-Spectrum-β-Lactamase-Producing Escherichia coli

机译：产生广谱β-内酰胺酶的大肠杆菌的SpectraCell RA分型和多基因座序列分型的比较

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Multilocus sequence typing (MLST) is one of the most reliable methods for typing of Escherichia coli, including extended-spectrum-β-lactamase-producing E. coli (ESBL-EC). We investigated the performance of a new typing method, SpectraCell RA (River Diagnostics, Madison, WI), in comparison on MLST on a well-defined collection of ESBL-EC isolates obtained from chicken meat and humans. Ninety-two ESBL-EC isolates obtained from meat and 59 ESBL-EC isolates obtained from human rectal swabs and clinical blood cultures were typed using MLST and SpectraCell RA. The sensitivity and specificity of SpectraCell RA were calculated, using MLST as a reference method. Subsequently, the results of SpectraCell RA were used to determine the relatedness of ESBL-EC isolates from chicken and humans. Using MLST as the gold standard, the performance of SpectraCell RA was evaluated for 3 different cutoff values: 0.99975, 0.99955, and 0.99935. Depending on the cutoff value, the sensitivity was mediocre to unacceptably low, with values of 9.4%, 43.9%, and 66.7%, respectively. When sensitivity increased, the specificity decreased rapidly, from 95.6% to 69.8% and 34.4%, respectively. Also, the number of clusters containing both human and meat samples varied from 0 (0.0%) to 14 (38.9%). Our study shows that SpectraCell RA is not a suitable typing method for ESBL-EC when evaluating relationships of ESBL-EC at the population level.
机译：多基因座序列分型（MLST）是最可靠的大肠杆菌分型方法之一，包括产生广谱β-内酰胺酶的大肠杆菌（ESBL-EC）。我们比较了从鸡和人获得的ESBL-EC分离株的明确定义上，在MLST上进行了比较，研究了新的分型方法SpectraCell RA（River Diagnostics，麦迪逊，威斯康星州）的性能。使用MLST和SpectraCell RA对来自肉类的92种ESBL-EC分离株和从人直肠拭子和临床血液培养物中分离的59种ESBL-EC分离株进行分型。使用MLST作为参考方法，计算SpectraCell RA的敏感性和特异性。随后，使用SpectraCell RA的结果确定了来自鸡和人的ESBL-EC分离株的相关性。使用MLST作为金标准，针对3个不同的截断值（0.99975、0.99955和0.99935）评估了SpectraCell RA的性能。取决于临界值，灵敏度中等至不可接受的低，分别为9.4％，43.9％和66.7％。当敏感性增加时，特异性迅速下降，分别从95.6％降至69.8％和34.4％。同样，包含人类和肉类样品的簇的数量从0（0.0％）到14（38.9％）不等。我们的研究表明，在人群水平评估ESBL-EC的关系时，SpectraCell RA不是适合ESBL-EC的分型方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号