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首页> 外文期刊>Journal of Clinical Microbiology >Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus
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Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus

机译：TaqMan阵列卡的开发，用于急性发热疾病暴发调查和新兴病原体（包括埃博拉病毒）的监测

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Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (Bartonella spp., Brucella spp., Coxiella burnetii, Leptospira spp., Rickettsia spp., Salmonella enterica and Salmonella enterica serovar Typhi, and Yersinia pestis), and 3 protozoa (Leishmania spp., Plasmodium spp., and Trypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.
机译：急性发热性疾病（AFI）与全球范围内的高发病率和死亡率相关，但通常并未发现病因。恢复期血清学检查不切实际，血液培养缓慢，许多病原体难以辨认或无法培养。我们开发了基于PCR的实时TaqMan阵列卡（TAC），可以在从样品到结果的2.5小时内测试六到八个样品，并且可以同时检测26种与AFI相关的生物，包括15种病毒（基孔肯雅热，克里米亚-刚果出血热）。 [CCHF]病毒，登革热，埃博拉病毒，Bundibugyo病毒，苏丹病毒，汉坦病毒[汉坦和汉城]，戊型肝炎，马尔堡，尼帕病毒，o'nyong-nyong病毒，纵谷热病毒，西尼罗河病毒和黄热病病毒），8种细菌（Bartonella spp。，布鲁氏菌（Brucella spp。），Coxiella burnetii，钩端螺旋体（Lepttospira spp。），立克次氏菌（Rickettsia spp。），肠炎沙门氏菌（Salmonella enterica）和肠炎沙门氏菌（Salmonella enterica serovar Typhi）和鼠疫耶尔森菌（Yersiniapestis）和3种原生动物（Leishmania spp。，疟原虫（Plasmodium spp。和布氏锥虫（Trypanosoma brucei）。包括两个外部对照（光疱疹病毒1和噬菌体MS2）以确保提取和扩增效率。在加标样品上进行线性，测定内精密度，测定间精密度，检测极限和特异性的分析验证。通过与单独的实时PCR分析相比较，用1,050个血液样本评估了卡片在临床样本上的性能，TAC总体显示为88％（278/315; 95％置信区间[CI]，从84％到92） ％）的敏感性和99％（5,261 / 5,326，98％至99％）的特异性。这款TaqMan阵列卡可以在现场设置中用作快速屏幕，以进行暴发调查或监视病原体，包括埃博拉病毒。

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