Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology

JiYoung Hong; Byunghak Kang; Ahyoun Kim; Seoyoun Hwang; Sunwha Lee; Jonghyen Kim; Hyun-Young Lee; Sang-Hyeon Kang; Doo-Sung Cheon

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Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology

机译：使用互补锁定引物技术的逆转录-PCR增强临床样品中肠病毒的检测

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To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

机译：为了提高检测灵敏度，我们使用互补锁定引物（CLP）技术修饰了引物。使用CLP修饰引物的逆转录PCR（RT-PCR）的敏感性比不使用这些引物的RT-PCR的敏感性高10到100倍。 CLP修饰的引物可以提高灵敏度，为分子诊断提供了一种广泛使用的方法。

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《Journal of Clinical Microbiology》 |2010年第2期|共2页
作者
JiYoung Hong; Byunghak Kang; Ahyoun Kim; Seoyoun Hwang; Sunwha Lee; Jonghyen Kim; Hyun-Young Lee; Sang-Hyeon Kang; Doo-Sung Cheon;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Comparison of a Reporter Assay and Immunomagnetic Separation Real-Time Reverse Transcription-PCR for the Detection of Enteroviruses in Seeded Environmental Water Samples [J] . Yu-Chen Hwang, Oymon M. Leong, Wilfred Chen, Applied and Environmental Microbiology . 2007,第7期

机译：记者分析和免疫磁分离实时逆转录PCR检测种子环境水样品中肠病毒的比较
2. Direct Detection of Enterovirus 71 (EV71) in Clinical Specimens from a Hand, Foot, and Mouth Disease Outbreak in Singapore by Reverse Transcription-PCR with Universal Enterovirus and EV71-Specific Primers [J] . Sunita Singh, Vincent T. K. Chow, M. C. Phoon, Journal of Clinical Microbiology . 2002,第8期

机译：通过通用肠病毒和EV71特异性引物的逆转录PCR直接检测新加坡手足口病暴发中临床样本中的肠道病毒71（EV71）
3. Evaluation of a Real-Time Reverse Transcription-PCR Assay for Detection of Enterovirus D68 in Clinical Samples from an Outbreak in New York State in 2014 [J] . Jian Zhuge, Eric Vail, Jeffrey L. Bush, Journal of Clinical Microbiology . 2015,第6期

机译：2014年纽约州暴发的临床样本中实时肠道逆转录-PCR分析检测肠病毒D68的评估
4. Development of a Quantitative Sequence Detection (QSD) Assay for Enteroviruses using TaqMan 7700 Technology [C] . Morteza Abbaszadegan, George Di Giovanni Water quality technology conference;WQTC . 1999

机译：使用TaqMan 7700技术开发肠病毒的定量序列检测（QSD）分析方法
5. Interactions between HIV-1 reverse transcriptase and chain-terminated primer/template: Discovery of a novel primer unblocking activity involved in resistance to AZT. [D] . Meyer, Peter Robert. 1999

机译：HIV-1逆转录酶和链终止引物/模板之间的相互作用：发现了一种新型的引物解封闭活性，涉及对AZT的抗性。
6. Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology [O] . JiYoung Hong, Byunghak Kang, Ahyoun Kim, 2010

机译：使用互补锁定引物技术的逆转录-PCR增强临床样品中肠病毒的检测
7. Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology▿ [O] . Hong, JiYoung, Kang, Byunghak, Kim, Ahyoun, 2009

机译：使用互补锁定引物技术的逆转录-PCR增强临床样品中肠病毒的检测▿

Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology

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