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首页> 外文期刊>Journal of Clinical Microbiology >Evolutionary Changes Affecting Rapid Identification of 2008 Newcastle Disease Viruses Isolated from Double-Crested Cormorants
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Evolutionary Changes Affecting Rapid Identification of 2008 Newcastle Disease Viruses Isolated from Double-Crested Cormorants

机译：进化变化影响快速识别从双冠Cor分离的2008年新城疫病毒。

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A morbidity-mortality event involving virulent Newcastle disease virus (NDV) in wild double-crested cormorants (Phalacrocorax auritus) occurred in North America in the summer of 2008. All 22 viruses isolated from cormorants were positively identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gene. However, the USDA-validated reverse transcription-PCR assay targeting the fusion gene that is specific for virulent isolates identified only 1 of these 22 isolates. Additionally, several of these isolates have been sequenced, and this information was used to identify genomic changes that caused the failure of the test and to revisit the evolution of NDV in cormorants. The forward primer and fusion probe were redesigned from the 2008 cormorant isolate sequence, and the revised fusion gene test successfully identified all 22 isolates. Phylogenetic analyses using both the full fusion sequence and the partial 374-nucleotide sequence identified these isolates as genotype V, with their nearest ancestor being an earlier isolate collected from Nevada in 2005. Histopathological analysis of this ancestral strain revealed morphological changes in the brain consistent with that of the traditional mesogenic pathotypes in cormorants. Intracerebral pathogenicity assays indicated that each of these isolates is virulent with values of >0.7 but not more virulent than earlier isolates reported from Canada.
机译：2008年夏季，在北美的野生双冠-（ Phalacrocorax auritus ）中发生了涉及致命性新城疫病毒（NDV）的发病率-死亡率事件。 USDA验证的针对基质基因的实时逆转录PCR分析。但是，针对有毒分离物特异的融合基因的USDA验证的逆转录PCR方法仅鉴定出这22种分离物中的一种。此外，已对其中的几种分离株进行了测序，并将此信息用于鉴定导致测试失败的基因组变化，并重新评估N中NDV的进化。从2008年mor分离株序列中重新设计了正向引物和融合探针，修订后的融合基因测试成功鉴定了所有22个分离株。使用完整融合序列和374个核苷酸的部分序列进行系统进化分析，确定这些分离株为基因型V，其最接近的祖先是2005年从内华达州采集的较早分离株。对该祖先菌株的组织病理学分析显示，脑部形态学变化与cor的传统介观型。脑内致病性分析表明，这些分离株均具有强毒力，其值> 0.7，但比加拿大报道的早期分离株强。

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