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首页> 外文期刊>Journal of Clinical Microbiology >Distribution of Nontuberculous Mycobacteria by Multigene Sequence-Based Typing and Clinical Significance of Isolated Strains
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Distribution of Nontuberculous Mycobacteria by Multigene Sequence-Based Typing and Clinical Significance of Isolated Strains

机译：基于多基因序列分型的非结核分枝杆菌的分布及其分离株的临床意义

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Species identification of nontuberculous mycobacteria (NTM) is challenging due to the increasing number of identified NTM species and the lack of standardized testing strategies. The objectives of this study were to investigate the distribution of NTM species recovered from respiratory specimens by multigene sequence-based typing and to evaluate the clinical significance of identified species. Two hundred thirty-two consecutive clinical NTM isolates were subjected to sequencing of multiple genes, including hsp65, rpoB, and 16S-23S rRNA internal transcribed spacer (ITS) sequence. In addition, clinical data from all patients whose specimens had NTM isolates were analyzed to examine clinical virulence and treatment history. Eighteen strains from 227 isolates from 169 patients were successfully identified at the species level by multigene sequence-based typing. Mycobacterium avium complex and M. abscessus complex made up the majority of isolated NTM (88%; 199/227), followed by M. fortuitum complex (4%; 10/227). The pathogenic potential of NTM differs enormously by species, and M. avium complex and M. abscessus complex revealed especially high levels of virulence compared with findings for other NTM species. The results from our work support M. avium complex and M. abscessus complex being the most common NTM species with highly pathogenic potential isolated from clinical respiratory specimens and could be a good resource for molecular epidemiology of NTM species in South Korea.
机译：非结核分枝杆菌（NTM）的物种鉴定具有挑战性，因为已鉴定的NTM物种数量不断增加，并且缺乏标准化的测试策略。这项研究的目的是调查通过基于多基因序列的分型从呼吸道标本中回收的NTM物种的分布，并评估已鉴定物种的临床意义。对322个连续的临床NTM分离株进行了多个基因测序，包括 hsp65 ， rpoB 和16S-23S rRNA内部转录间隔区（ITS）序列。此外，分析了所有标本具有NTM分离物的患者的临床数据，以检查临床毒力和治疗史。通过基于多基因序列的分型成功地在物种水平上鉴定了来自169名患者的227个分离株的18个菌株。鸟分枝杆菌复合物和脓肿支原体复合物占分离出的NTM的大部分（88％; 199/227），其次是福特支原体复合物（4％; 10/227）。 NTM的致病潜力因物种而异，并且与其他NTM物种相比，鸟分枝杆菌复合体和脓肿分枝杆菌复合体显示出特别高的毒力。我们的工作结果支持鸟形支原体复合体和脓肿性支原体复合体是从临床呼吸道标本中分离出的最具致病性的最常见NTM物种，并且可能是韩国NTM物种分子流行病学的良好资源。

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