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首页> 外文期刊>Journal of cell biology >Submicromolar levels of calcium control the balance of beating between the two flagella in demembranated models of Chlamydomonas.
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Submicromolar levels of calcium control the balance of beating between the two flagella in demembranated models of Chlamydomonas.

机译：钙的超微摩尔水平控制着衣原体去膜模型中两个鞭毛之间的跳动平衡。

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When detergent-extracted, demembranated cell models of Chlamydomonas were resuspended in reactivation solutions containing less than 10(-8) M Ca++, many models initially swam in helical paths similar to those of intact cells; others swam in circles against the surface of the slide or coverslip. With increasing time after reactivation, fewer models swam in helices and more swam in circles. This transition from helical to circular swimming was the result of a progressive inactivation of one of the axonemes; in the extreme case, one axoneme was completely inactive whereas the other beat with a normal waveform. At these low Ca++ concentrations, the inactivated axoneme was the trans-axoneme (the one farthest from the eyespot) in 70-100% of the models. At 10(-7) or 10(-6) M Ca++, cell models also proceeded from helical to circular swimming as a result of inactivation of one of the axonemes; however, under these conditions the cis-axoneme was usually the one that was inactivated. At 10(-8) M Ca++, most cells continued helical swimming, indicating that both axonemes were remaining relatively active. The progressive, Ca++-dependent inactivation of the trans- or cis-axoneme was reversed by switching the cell models to higher or lower Ca++ concentrations, respectively. A similar reversible, selective inactivation of the trans-flagellum occurred in intact cells swimming in medium containing 0.5 mM EGTA and no added Ca++. The results show that there are functional differences between the two axonemes of Chlamydomonas. The differential responses of the axonemes to submicromolar concentrations of Ca++ may form the basis for phototactic turning.
机译：当用去污剂提取的衣藻的去膜细胞模型重悬于含有少于10（-8）M Ca ++的活化溶液中时，许多模型最初会以与完整细胞相似的螺旋路径游动。其他人则在幻灯片或盖玻片的表面游动。随着重新激活后时间的增加，越来越少的模型游动成螺旋形，而更多的游动成环。从螺旋形游泳到圆形游泳的转变是轴突之一逐渐失活的结果。在极端情况下，一个轴突完全不活动，而另一个则以正常波形跳动。在这些低Ca ++浓度下，在70％至100％的模型中，灭活的轴突酶是反式轴突酶（距眼点最远的一个）。在10（-7）或10（-6）M Ca ++时，由于一种轴突失活，细胞模型也从螺旋游动变为环形游动。然而，在这些条件下，顺式-轴突酶通常是被灭活的。在10（-8）M Ca ++时，大多数细胞继续螺旋游动，表明两个轴突均保持相对活跃。通过将细胞模型分别转换为更高或更低的Ca ++浓度，可以逆转反式或顺式-轴心酶的渐进性Ca ++依赖性失活。在含有0.5 mM EGTA且未添加Ca ++的培养基中游泳的完整细胞中，发生了类似的可逆性，选择性的失活。结果表明衣藻的两个轴突之间存在功能差异。轴突对亚微摩尔浓度的Ca ++的不同反应可能构成光战术转向的基础。

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