...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Resolution of a Protracted Serogroup B Meningococcal Outbreak with Whole-Genome Sequencing Shows Interspecies Genetic Transfer
【24h】

Resolution of a Protracted Serogroup B Meningococcal Outbreak with Whole-Genome Sequencing Shows Interspecies Genetic Transfer

机译：全基因组测序解决了旷日持久的血清B型脑膜炎球菌暴发，表明种间遗传转移。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A carriage study was undertaken (n = 112) to ascertain the prevalence of Neisseria spp. following the eighth case of invasive meningococcal disease in young children (5 to 46 months) and members of a large extended indigenous ethnic minority Traveller family (n = 123), typically associated with high-occupancy living conditions. Nested multilocus sequence typing (MLST) was employed for case specimen extracts. Isolates were genome sequenced and then were assembled de novo and deposited into the Bacterial Isolate Genome Sequencing Database (BIGSdb). This facilitated an expanded MLST approach utilizing large numbers of loci for isolate characterization and discrimination. A rare sequence type, ST-6697, predominated in disease specimens and isolates that were carried (n = 8/14), persisting for at least 44 months, likely driven by the high population density of houses (n = 67/112) and trailers (n = 45/112). Carriage for Neisseria meningitidis (P < 0.05) and Neisseria lactamica (P < 0.002) (2-sided Fisher's exact test) was more likely in the smaller, more densely populated trailers. Meningococcal carriage was highest in 24- to 39-year-olds (45%, n = 9/20). Evidence of horizontal gene transfer (HGT) was observed in four individuals cocolonized by Neisseria lactamica and Neisseria meningitidis. One HGT event resulted in the acquisition of 26 consecutive N. lactamica alleles. This study demonstrates how housing density can drive meningococcal transmission and carriage, which likely facilitated the persistence of ST-6697 and prolonged the outbreak. Whole-genome MLST effectively distinguished between highly similar outbreak strain isolates, including those isolated from person-to-person transmission, and also highlighted how a few HGT events can distort the true phylogenetic relationship between highly similar clonal isolates.
机译：进行了一项马车研究（ n = 112），以确定奈瑟氏球菌的患病率。在幼儿（5至46个月）的第八例侵袭性脑膜炎球菌病和大型原住民少数民族旅行者家庭（ n = 123）的成员中，通常与高居住条件相关。巢式多基因座序列分型（MLST）用于病例标本提取。对分离物进行基因组测序，然后从头开始进行组装，并保存到细菌分离物基因组测序数据库（BIGSdb）中。这促进了扩展的MLST方法的应用，该方法利用了大量的基因座进行了分离特征和鉴别。一种罕见的序列类型ST-6697，主要携带在疾病标本和分离株中（ n = 8/14），可持续至少44个月，这可能是由于房屋人口密度高造成的（ n = 67/112）和预告片（ n = 45/112）。脑膜炎奈瑟氏球菌（ P <0.05）和乳酸奈瑟氏球菌（ P <0.002）（两面费舍尔精确检验）的运输更可能在较小，人口稠密的拖车中进行。脑膜炎球菌携带率最高的是24岁至39岁（45％， n = 9/20）。在由乳酸奈瑟氏菌和脑膜炎奈瑟氏菌共定殖的四个个体中观察到水平基因转移（HGT）的证据。一项HGT事件导致获得了26个连续的乳酸奈瑟球菌等位基因。这项研究证明了住房密度如何驱动脑膜炎球菌的传播和运输，这可能促进了ST-6697的持久性并延长了爆发时间。全基因组MLST有效区分了高度相似的暴发菌株，包括从人与人之间传播的菌株，并强调了一些HGT事件如何扭曲高度相似的克隆菌株之间的真实系统发育关系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号