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首页> 外文期刊>Journal of Clinical Microbiology >Screening for Acinetobacter baumannii Colonization by Use of Sponges
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Screening for Acinetobacter baumannii Colonization by Use of Sponges

机译：用海绵筛选鲍曼不动杆菌定植

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There is currently no consensus method for the active screening of Acinetobacter baumannii. The use of swabs to culture nostrils, pharynx, and skin surface of various anatomical sites is known to yield less-than-optimal sensitivity. In the present study, we sought to determine whether the use of sterile sponges to sample large areas of the skin would improve the sensitivity of the detection of A. baumannii colonization. Forty-six patients known to be colonized with A. baumannii, defined by a positive clinical culture for this organism as defined by resistance to more than two classes of antimicrobials, participated in the study. The screening sites included the forehead, nostrils, buccal mucosa, axilla, antecubital fossa, groin, and toe webs with separate rayon swabs and the forehead, upper arm, and thigh with separate sponges. Modified Leeds Acinetobacter medium (mLAM) agar plates that contained vancomycin and either aztreonam or ceftazidime were used as the selective medium. An enrichment culture grown overnight substantially increased the sensitivity for most sites. The sensitivity ranged between 69.6 and 82.6% for individual sponge sites and 21.7 to 52.2% for individual swab sites when mLAM plates with ceftazidime were inoculated after a 24-h enrichment period. The sponge and swab sites with the best sensitivity were the leg and the buccal mucosa, respectively (82.6% and 52.2%; P = 0.003). The combined sensitivity for the upper arm and leg with a sponge was 89.1%. The novel screening method using sterile sponges was easy to perform and achieved excellent sensitivity for the detection of A. baumannii colonization.
机译：目前尚无用于主动筛选鲍曼不动杆菌的共识方法。众所周知，使用拭子培养鼻孔，咽部和各种解剖部位的皮肤表面会产生低于最佳灵敏度的现象。在本研究中，我们试图确定使用无菌海绵对大面积皮肤进行采样是否会提高 A检测的灵敏度。鲍曼氏菌定殖。已知有46名患者被定植为 A。鲍曼氏菌是由该生物体的阳性临床培养物定义的，该细菌由对两种以上抗菌素的抗性定义。筛查部位包括额头，鼻孔，颊粘膜，腋窝，肘前窝，腹股沟和带有独立人造丝拭子的脚趾网，以及额头，上臂和大腿用独立的海绵。包含万古霉素和氨曲南或头孢他啶的改良利兹不动杆菌培养基（mLAM）琼脂平板用作选择性培养基。过夜生长的富集培养物大大提高了大多数部位的敏感性。在富集24小时后接种带有头孢他啶的mLAM平板时，单个海绵位点的敏感性范围为69.6至82.6％，单个拭子位点的敏感性范围为21.7至52.2％。敏感性最高的海绵和拭子部位分别是腿部和颊粘膜（分别为82.6％和52.2％; P = 0.003）。上臂和腿部海绵的综合敏感性为89.1％。使用无菌海绵的新型筛选方法易于操作，并且对 A的检测具有出色的灵敏度。鲍曼氏菌定殖。

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