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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Bactec Mycosis IC/F and Plus Aerobic/F Blood Culture Bottles for Detection of Candida in the Presence of Antifungal Agents
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Evaluation of Bactec Mycosis IC/F and Plus Aerobic/F Blood Culture Bottles for Detection of Candida in the Presence of Antifungal Agents

机译：评估使用Bactec真菌病IC / F和Plus有氧/ F血培养瓶检测是否存在抗真菌剂的念珠菌

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Clinical practice guidelines recommend performing follow-up cultures for patients with candidemia in order to determine the time when Candida is cleared from the bloodstream. Since this requires culturing blood samples from patients undergoing antifungal treatment, we evaluated two blood culture bottles (the Bactec Mycosis IC/F [MICF], specifically adapted to the growth of fungi, and the Bactec Plus Aerobic/F [PAF], containing resins to inactivate anti-infective agents) for their effectiveness in detecting Candida albicans and Candida glabrata when seeded in concentrations of 1 CFU/ml and 10 CFU/ml, respectively, together with human whole blood and various antifungal agents in therapeutic peak serum concentrations (Cmax). Significant differences between the MICF and PAF vials for the detection of Candida spp. were found when inoculated with caspofungin (0/12 versus 8/12) (P < 0.001) or amphotericin B (3/12 versus 12/12) (P < 0.001). Inoculation of fluconazole or voriconazole did not influence the effectiveness of detection in the MICF and PAF bottles (P = 1.0). Neither the MICF nor the PAF bottles detected Candida spp. reliably when seeded together with anidulafungin (1/12 versus 1/12) (P = 1.0) or micafungin (0/12 versus 1/12) (P = 1.0). The times to positivity of both bottles were significantly prolonged when antifungal agents were added compared to those of controls without antimycotic drugs (P < 0.001). Overall, the results of this in vitro study indicate that the PAF bottles detected Candida spp. more reliably than the MICF bottles when supplemented with certain antifungal agents. Consequently, clinical studies should evaluate whether this holds true when blood cultures from patients undergoing antifungal treatment are performed.
机译：临床实践指南建议对念珠菌血症患者进行随访培养，以确定从血液中清除念珠菌的时间。由于这需要从接受抗真菌治疗的患者中采集血样，因此我们评估了两个血液培养瓶（专门用于真菌生长的Bactec Mycosis IC / F [MICF]和含有树脂的Bactec Plus好氧/ F [PAF]灭活抗感染剂），分别以1 CFU / ml和10 CFU / ml的浓度接种人白血球菌和gladata光滑念珠菌，以及治疗峰值血清浓度的人全血和各种抗真菌剂（< em> C max ）。 MICF和PAF小瓶在检测假丝酵母中的显着差异。被卡泊芬净（0/12对8/12）（ P <0.001）或两性霉素B（3/12对12/12）（ P <0.001）接种时被发现）。接种氟康唑或伏立康唑不会影响MICF和PAF瓶中的检测效果（ P = 1.0）。 MICF和PAF瓶均未检测到假丝酵母。与阿地芬净（1/12对1/12）（ P = 1.0）或米卡芬净（0/12对1/12）（ P = 1.0）一起可靠播种。与未添加抗真菌药的对照组相比，添加抗真菌剂后，两个瓶子的阳性反应时间均显着延长（ P <0.001）。总体而言，这项体外研究的结果表明，PAF瓶检测到了假丝酵母菌。当补充某些抗真菌剂时，比MICF瓶更可靠。因此，临床研究应评估在接受抗真菌治疗的患者进行血液培养时是否成立。

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