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首页> 外文期刊>Journal of Clinical Microbiology >Phenotypic and Genotypic Changes over Time and across Facilities of Serial Colonizing and Infecting Escherichia coli Isolates Recovered from Injured Service Members
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Phenotypic and Genotypic Changes over Time and across Facilities of Serial Colonizing and Infecting Escherichia coli Isolates Recovered from Injured Service Members

机译：从受伤的服务成员中回收的随时间推移和跨设施的一系列定殖和感染大肠杆菌分离株的表型和基因型变化

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Escherichia coli is the most common colonizing and infecting organism isolated from U.S. service members injured during deployment. Our objective was to evaluate the phenotypic and genotypic changes of infecting and colonizing E. coli organisms over time and across facilities to better understand their transmission patterns. E. coli isolates were collected via surveillance cultures and infection workups from U.S. military personnel injured during deployment (June 2009 to May 2011). The isolates underwent antimicrobial susceptibility testing, pulsed-field gel electrophoresis, and multiplex PCR for phylotyping to determine their resistance profiles and clonality. A total of 343 colonizing and 136 infecting E. coli isolates were analyzed, of which 197 (57%) and 109 (80%) isolates, respectively, produced extended-spectrum β-lactamases (ESBL). Phylogroup A was predominant among both colonizing (38%) and infecting isolates (43%). Although 188 unique pulsed-field types (PFTs) were identified from the colonizing isolates, and 54 PFTs were identified from the infecting isolates, there was a lack of PFT overlap between study years, combat zones, and military treatment facilities. On a per-subject basis, 26% and 32% of the patients with serial colonizing isolates and 10% and 21% with serial infecting isolates acquired changes in their phylogroup and PFT profiles, respectively, over time. The production of ESBL remained high over time and across facilities, with no substantial changes in antimicrobial susceptibilities. Overall, our results demonstrated an array of genotypic and phenotypic differences for the isolates without large clonal clusters; however, the same PFTs were occasionally observed in the colonizing and infecting isolates, suggesting that the source of infections may be endogenous host organisms.
机译：大肠杆菌是从部署期间受伤的美国军人中分离出来的最常见的定殖和感染性生物。我们的目标是评估一段时间内和跨设施感染和定殖的大肠杆菌生物的表型和基因型变化，以更好地了解其传播方式。通过监视培养和感染检查从部署期间受伤的美国军事人员（2009年6月至2011年5月）收集了大肠杆菌分离株。这些分离物经过了抗菌药敏测试，脉冲场凝胶电泳和多重PCR进行系统分型，以确定其耐药性和克隆性。共分析了343个定殖和136个感染大肠杆菌分离株，其中分别有197个（57％）和109个（80％）分离株产生了广谱β-内酰胺酶（ESBL）。 Phylogroup A在定居（38％）和感染分离株（43％）中均占主导地位。尽管从定殖的菌株中鉴定出了188种独特的脉冲场类型（PFT），而从感染的菌株中鉴定出了54种PFT，但在研究年份，作战区域和军事治疗设施之间缺乏PFT重叠。在每个受试者的基础上，分别有26％和32％的具有连续定殖分离株的患者以及10％和21％的具有连续感染性分离株的患者随着时间的推移分别获得了系统群和PFT谱的变化。随着时间的推移和整个设施的生产，ESBL的产量仍然很高，而抗菌药的敏感性没有实质性的变化。总体而言，我们的结果表明，没有较大克隆簇的分离株在基因型和表型上存在差异。然而，在定殖和感染的分离株中偶尔会观察到相同的PFT，这表明感染源可能是内源性宿主生物。

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