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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Epidemiology and Genetic Diversity of Fluoroquinolone-Resistant Escherichia coli Isolates from Patients with Community-Onset Infections in 30 Chinese County Hospitals
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Molecular Epidemiology and Genetic Diversity of Fluoroquinolone-Resistant Escherichia coli Isolates from Patients with Community-Onset Infections in 30 Chinese County Hospitals

机译：中国30所县医院社区感染患者氟喹诺酮耐药菌株的分子流行病学和遗传多样性

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The high frequency of fluoroquinolone resistance in Escherichia coli is a feature of clinical bacteriology in China, where the molecular epidemiology and genetic characteristics of this resistance in county hospitals remain unclear. A total of 590 nonduplicate E. coli isolates from 30 county hospitals located across seven Chinese regions were examined for plasmid-mediated quinolone resistance (PMQR) genes and mutations in quinolone resistance-determining regions (QRDRs). Multilocus sequence typing (MLST) and phylogenetic analysis of fluoroquinolone-resistant isolates were used to determine their genetic relatedness. The ciprofloxacin resistance rate of community-onset E. coli was 51.2%, and at least one PMQR gene was carried by 220 (37.3%) isolates. These included qnr (3.7%), aac(6′)-Ib-cr (19.7%), qepA (14.4%), and oqxAB (3.8%). Two novel oqxB mutants were identified and named oqxB20 and oqxB29. From 60 sequence types (STs) isolated, 5 novel STs (ST4499 to ST4503) were identified. ST1193 (7.9%) was the second most abundant ST among fluoroquinolone-resistant isolates (ST131 was the most common, with 14.6%), and this is the first report of it in China. This is also the first report of ST2115 and ST3014 isolates from human samples. Ciprofloxacin-resistant E. coli isolates fell mainly into phylogroups B2 and D. The rates of fluoroquinolone resistance and the prevalence of PMQR genes in community-onset E. coli isolates from Chinese county hospitals were high. The wide-ranging molecular epidemiology of E. coli isolates from scattered locations across China indicates that fluoroquinolone resistance evolved from different sources.
机译：大肠杆菌对氟喹诺酮耐药的高频率是中国临床细菌学的特征，县医院对这种耐药的分子流行病学和遗传特征尚不清楚。考察了来自中国7个地区的30家县医院的590例非重复性大肠杆菌分离株的质粒介导的喹诺酮抗性（PMQR）基因和喹诺酮抗性决定区域（QRDRs）的突变。耐氟喹诺酮分离株的多基因座序列分型（MLST）和系统发育分析用于确定其遗传相关性。社区感染大肠埃希菌对环丙沙星的耐药率为51.2％，并且220个（37.3％）分离株携带至少一个PMQR基因。其中包括 qnr （3.7％）， aac（6 '）-Ib-cr （19.7％）， qepA （14.4％）和 oqxAB （3.8％）。鉴定了两个新颖的 oqxB 突变体，分别命名为 oqxB20 和 oqxB29 。从分离的60个序列类型（ST）中，鉴定出5个新的ST（ST4499至ST4503）。 ST1193（7.9％）是氟喹诺酮耐药菌株中第二大的ST（ST131最常见，占14.6％），这是它在中国的首次报道。这也是人类样品中ST2115和ST3014分离株的首次报道。耐环丙沙星的大肠埃希菌菌株主要分为B2和D组。中国县医院社区感染大肠埃希菌中氟喹诺酮耐药率和PMQR基因普遍存在。遍布中国各地的大肠埃希菌的广泛分子流行病学表明，氟喹诺酮耐药性来自不同来源。

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