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Efficient Isolation of Swine Influenza Viruses by Age-Targeted Specimen Collection

机译：以年龄为目标的标本采集有效分离猪流感病毒

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The control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain “undiscovered” in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection.
机译：猪流感病毒（SIV）感染的控制对于提高养猪业的生产率和最大程度地减少大流行性疾病爆发的威胁至关重要。因此，SIV监视应按地区定期进行。在这里，我们通过使用表达报告基因的重组流感病毒建立了针对SIV血清流行性监测的微中和试验。基于生长的SIV血清阳性率表明，大多数母猪和仔猪中和抗流感病毒的抗体呈阳性。相反，90天大的生长猪在其血清中显示出有限的中和活性，表明该特定年龄的猪群最容易感染SIV，因此是分离SIV的理想年龄组。从这个年龄段的健康猪的鼻拭子样本中，我们能够以比以前的报告更高的发病率（5.3％）分离出SIV。血凝素（HA）基因的核苷酸测序和系统发育分析表明，分离的SIV已在猪中循环和进化，但最近尚未从人体内引入，这意味着全球猪种群中可能仍“未发现”大量SIV谱系。我们建议在这项研究中提出的90天大的以猪为目标的鼻拭子集合有助于全球SIV监测，并有助于SIV感染的检测和控制。

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