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首页> 外文期刊>Journal of Clinical Microbiology >Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil
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Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil

机译：拉丁美洲地中海谱系的耐多药结核分枝杆菌，被错误地鉴定为pinnipedii分支杆菌（Spoligotype International Type 863 [SIT863]），在巴西南部引起活动性结核病

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We recently detected the spoligotype patterns of strains of Mycobacterium pinnipedii, a species of the Mycobacterium tuberculosis complex, in sputum samples from nine cases with pulmonary tuberculosis residing in Porto Alegre, South Brazil. Because this species is rarely encountered in humans, we further characterized these nine isolates by additional genotyping techniques, including 24-locus mycobacterial interspersed repetitive-unit–variable-number tandem-repeat (MIRU-VNTR) typing, verification of the loci TbD1, RD9, pks15/1, RDRio, and fbpC, the insertion of IS6110 at a site specific to the M. tuberculosis Latin American Mediterranean (LAM) lineage, and whole-genome sequencing. The combined analysis of these markers revealed that the isolates are in fact M. tuberculosis and more specifically belong to the LAM genotype. Most of these isolates (n = 8) were shown to be multidrug resistant (MDR), which prompted us to perform partial sequencing of the rpoA, rpoB, rpoC, katG, and inhA genes. Seven isolates (77.8%) carried the S315T mutation in katG, and one of these (11%) also presented the C(?17)T single-nucleotide polymorphism (SNP) in inhA. Interestingly, six of the MDR isolates also presented an undescribed insertion of 12 nucleotides (CCA GAA CAA CCC) in codon 516 of rpoB. No putative compensatory mutation was found in either rpoA or rpoC. This is the first report of an M. tuberculosis LAM family strain with a convergent M. pinnipedii spoligotype. These spoligotypes are observed in genotype databases at a modest frequency, highlighting that care must be taken when identifying isolates in the M. tuberculosis complex on the basis of single genetic markers.
机译：我们最近在巴西南部阿雷格里港的9例肺结核病例的痰液样本中检测到了Pinnipedii分支杆菌（一种结核分枝杆菌复合体）菌株的spoligotype模式。因为该物种在人类中很少见，所以我们通过其他基因分型技术进一步表征了这9种分离株，包括24位分枝杆菌散布的重复单位-可变数目串联重复序列（MIRU-VNTR）分型，基因座TbD1，RD9的验证， pks15 / 1 ，RD Rio 和 fbpC ，IS 6110 在特定于结核分枝杆菌拉丁美洲地中海（LAM）谱系和全基因组测序。对这些标记物的综合分析表明，分离物实际上是结核分枝杆菌，更具体地说，属于LAM基因型。其中大多数分离株（ n = 8）具有多重耐药性（MDR），这促使我们对 rpoA ， rpoB 进行了部分测序。 em>， rpoC ， katG 和 inhA 基因。七个分离株（77.8％）在 katG 中带有S315T突变，其中一个（11％）也在 inhA <中表现出C（？17）T单核苷酸多态性（SNP）。 / em>。有趣的是，其中的六个MDR分离株在 rpoB 的516位密码子中还出现了12个核苷酸的未描述插入（CCA GAA CAA CCC）。在 rpoA 或 rpoC 中均未发现任何代偿性突变。这是首次发现结核分枝杆菌LAM家族菌株具有趋于融合的pinnipedii spoligotype。这些spoligotypes在基因型数据库中以适度的频率观察到，突出表明在基于单个遗传标记物鉴定结核分枝杆菌复合体中的分离株时必须小心。

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