Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Ana Carolina Ramos; Cecília Godoy Carvalhaes; Jhonatha Rodrigo Cordeiro-Moura; Anna Carolina Rockstroh; Antonia Maria Oliveira Machado; Ana Cristina Gales

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Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

机译：培养基对基质辅助激光解吸电离-飞行时间质谱检测碳青霉烯水解的影响

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In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization–time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.

机译：在这项研究中，我们评估了不同细菌生长培养基对基质辅助激光解吸电离-飞行时间质谱检测碳青霉烯酶水解的影响。在MacConkey琼脂培养基上生长的OXA-25-，OXA-26-和OXA-72鲍曼不动杆菌分离株观察到假阴性结果。其他培养基显示出100％的敏感性和100％的特异性检测碳青霉烯酶。

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《Journal of Clinical Microbiology》 |2016年第7期|共3页
作者
Ana Carolina Ramos; Cecília Godoy Carvalhaes; Jhonatha Rodrigo Cordeiro-Moura; Anna Carolina Rockstroh; Antonia Maria Oliveira Machado; Ana Cristina Gales;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry [J] . Ana Carolina Ramos, Cecília Godoy Carvalhaes, Jhonatha Rodrigo Cordeiro-Moura, Journal of Clinical Microbiology . 2016,第7期

机译：培养基对基质辅助激光解吸电离-飞行时间质谱检测碳青霉烯水解的影响
2. Performance of Two Resin-Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems [J] . Barbara Fiori, Tiziana DInzeo, Viviana Di Florio, Journal of Clinical Microbiology . 2014,第10期

机译：两种含树脂的血液培养基在检测血流感染和直接基质辅助激光解吸电离中的性能–飞行时间质谱（MALDI-TOF MS）肉汤测定法用于分离物鉴定：BacT / Alert Plus和BacT / Alert Plus的临床比较Bactec Plus系统
3. Universal protocol for the rapid automated detection of carbapenem-resistant Gram-negative bacilli directly from blood cultures by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) [J] . Oviano Marina, Sparbier Katrin, Jose Barba Maria, International journal of antimicrobial agents . 2016,第6期

机译：通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF / MS）直接从血液培养物中快速自动检测对碳青霉烯耐药的革兰氏阴性杆菌的通用协议
4. Optimization of Sample Preparation for Detection of Common Food-borne Pathogens by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [C] . Lay Hoon Seah, Tze Horng Liew, Aldrich Leow, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：通过基质辅助激光解吸电离 - 飞行质谱法检测常见食品传播病原体的样品制剂的优化
5. Laser desorption/laser ionization time-of-flight mass spectrometry instrument design and investigation of the desorption and ionization mechanisms of matrix-assisted laser desorption/ionization. [D] . King, Richard Charles, III. 1994

机译：激光解吸/激光电离飞行时间质谱仪的设计以及基质辅助激光解吸/电离的解吸和电离机理研究。
6. Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry [O] . Ana Carolina Ramos, Cecília Godoy Carvalhaes, Jhonatha Rodrigo Cordeiro-Moura, 2016

机译：培养基对基质辅助激光解吸电离-飞行时间质谱检测碳青霉烯水解的影响
7. Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry [O] . Ana Carolina Ramos, Cecília Godoy Carvalhaes, Jhonatha Rodrigo Cordeiro-Moura, 2016

机译：培养培养培养培养物对碳烯烃水解检测的基质辅助激光解吸电离 - 飞行质谱时间

Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

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