...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species
【24h】

A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species

机译：一种改进的碳青霉烯灭活方法CIMTris，用于在不动杆菌属和假单胞菌属物种中产生碳青霉烯酶

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The carbapenem inactivation method (CIM) and modified CIM (mCIM) are simple and economical phenotypic screening methods for detecting carbapenemase production in Gram-negative bacteria. Although the mCIM has been recommended by the Clinical and Laboratory Standards Institute, both the CIM and mCIM have limitations. This study describes another modified CIM, called CIMTris, in which carbapenemase was extracted from bacteria with 0.5 M Tris-HCl (pH 7.6) buffer. The ability of the CIMTris to detect carbapenemase production was examined in Acinetobacter and Pseudomonas species. The CIMTris had an overall sensitivity of 97.6% and an overall specificity of 92.6%, whereas the mCIM had a sensitivity of 45.1% and a specificity of 100% for the isolates tested. These findings indicate that the CIMTris is useful for detecting carbapenemase production in Acinetobacter and Pseudomonas species.
机译：碳青霉烯灭活方法（CIM）和修饰的CIM（mCIM）是用于检测革兰氏阴性细菌中碳青霉烯酶产生的简单且经济的表型筛选方法。尽管临床和实验室标准协会推荐了mCIM，但CIM和mCIM都有局限性。这项研究描述了另一个改良的CIM，称为CIMTris，其中碳青霉烯酶是用0.5 M Tris-HCl（pH 7.6）缓冲液从细菌中提取的。在不动杆菌属和假单胞菌属物种中检查了CIMTris检测碳青霉烯酶产生的能力。 CIMTris的总敏感性为97.6％，总特异性为92.6％，而mCIM对测试的分离物的敏感性为45.1％，特异性为100％。这些发现表明，CIMTris可用于检测不动杆菌属和假单胞菌属物种中碳青霉烯酶的产生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号