...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Probability of Negative Mycobacterium tuberculosis Complex Cultures Based on Time to Detection of Positive Cultures: a Multicenter Evaluation of Commercial-Broth-Based Culture Systems
【24h】

Probability of Negative Mycobacterium tuberculosis Complex Cultures Based on Time to Detection of Positive Cultures: a Multicenter Evaluation of Commercial-Broth-Based Culture Systems

机译：基于时间的阴性结核分枝杆菌复杂培养物检测阳性培养物的可能性：基于商业肉汤的培养系统的多中心评估

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We conducted a multicenter study to determine whether Mycobacterium tuberculosis complex (MTBC) cultures in automated broth-based systems could reliably be considered negative sooner than 6 weeks. Laboratory sites used Bactec MGIT or BacT/Alert and tracked results of time to detection of all mycobacteria (TTD-all, n = 1547) and of MTBC (TTD-MTBC, n = 466) over 6-month periods from primarily (93%) respiratory specimens. Cumulative percentages by day detected and median TTD of initial and follow-up specimens were analyzed. The median TTD-MTBC for MGIT (n = 6 sites) was 14 days. For laboratories using standard processing procedures, 100% of MTBC were detected from initial and follow-up specimens in 28 and 35 days, respectively, and no yield of MTBC on solid or MGIT liquid media was observed after 5 weeks. The median TTD-MTBC for BacT/Alert (n = 3 sites) was 18 days, with 95% and 100% detected within 37 and 42 days, respectively. Analysis of TTD of positive MTBC cultures in broth can predict the probability of culture negativity at defined time points. Receipt of interim negative reports earlier than 6 weeks could assist clinicians in considering alternative diagnoses and could alter the timing and prioritization of public health interventions. Laboratories should analyze their own TTD data to inform protocol decisions. Laboratories using MGIT could issue reports of no growth of MTBC on initial specimens as early as 4 weeks and for patients undergoing treatment as early as 5 weeks postinoculation.
机译：我们进行了一项多中心研究，以确定基于自动肉汤的系统中的结核分枝杆菌复合物（MTBC）培养是否早于6周就可以可靠地视为阴性。实验室地点使用了Bactec MGIT或BacT / Alert，并跟踪了检测所有分枝杆菌（TTD-all， n = 1547）和MTBC（TTD-MTBC， n = 466）在六个月内主要来自（93％）呼吸道样本。分析了按天计算的累积百分比以及初始和随访样本的TTD中值。 MGIT（ n = 6个位点）的TTD-MTBC中位数为14天。对于使用标准处理程序的实验室，分别在28和35天内从初始样本和后续样本中检测到100％的MTBC，并且在5周后未观察到固体或MGIT液体培养基上MTBC的产率。 BacT / Alert（ n = 3个位点）的TTD-MTBC中位数为18天，分别在37和42天内检测到95％和100％。肉汤中MTBC阳性培养物的TTD分析可以预测在指定时间点培养物阴性的可能性。在6周之前收到中期阴性报告可以帮助临床医生考虑其他诊断方法，并且可以改变公共卫生干预措施的时间安排和优先次序。实验室应分析自己的TTD数据以指导方案决策。使用MGIT的实验室最早可在4周后发布原始样本的MTBC增长报告，而对于接种后5周内接受治疗的患者则无此报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号