Russian “Successful” Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening

Igor Mokrousov; Olga Narvskaya; Anna Vyazovaya; Tatiana Otten; Wei-Wei Jiao; Lia Lima Gomes

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Russian “Successful” Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening

机译：俄罗斯结核分枝杆菌北京基因型的“成功”克隆B0 / W148：用于快速检测和全局筛选的多重PCR分析

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We describe a multiplex PCR assay to detect the Mycobacterium tuberculosis Beijing genotype variant B0/W148, which is considered a “successful” clone of M. tuberculosis, widespread in Russia. Specificity and sensitivity of the assay were 100% based on the analysis of a collection of 516 M. tuberculosis isolates of different genotypes and origins. This assay may be used for accurate and simple detection and surveillance of this clinically and epidemiologically important variant of M. tuberculosis.

机译：我们描述了一种多重PCR检测法，以检测结核分枝杆菌北京基因型变异B0 / W148，这被认为是结核分枝杆菌的“成功”克隆，在俄罗斯流行。根据对不同基因型和来源的516株结核分枝杆菌分离物的分析，该方法的特异性和敏感性为100％。该测定法可用于对结核分枝杆菌的这一临床和流行病学上重要的变异体进行准确，简单的检测和监测。

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《Journal of Clinical Microbiology》 |2012年第11期|共3页
作者
Igor Mokrousov; Olga Narvskaya; Anna Vyazovaya; Tatiana Otten; Wei-Wei Jiao; Lia Lima Gomes;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Increased transmissibility of Russian successful strain Beijing B0/W148 of Mycobacterium tuberculosis: Indirect clues from history and demographics [J] . Vyazovaya Anna, Proshina Eugeniya, Gerasimova Alena, Tuberculosis . 2020,第1期

机译：增加俄罗斯成功菌株的传播性北京北京分枝杆菌的B0 / W148：历史和人口统计学的间接线索
2. Tuberculous Spondylitis in Russia and Prominent Role of Multidrug-Resistant Clone Mycobacterium tuberculosis Beijing B0/W148 [J] . Vyazovaya Anna, Mokrousov Igor, Solovieva Natalia, Antimicrobial agents and chemotherapy. . 2015,第4期

机译：俄罗斯结核性脊柱炎和耐多药结核分枝杆菌的重要作用北京B0 / W148
3. Mechanisms of Phenotypic Rifampicin Tolerance in Mycobacterium tuberculosis Beijing Genotype Strain B0/W148 Revealed by Proteomics [J] . de Keijzer Jeroen, Mulder Arnout, de Beer Jessica, Journal of proteome research . 2016,第4期

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6. Russian Successful Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening [O] . Igor Mokrousov, Olga Narvskaya, Anna Vyazovaya, 2012

机译：俄罗斯结核分枝杆菌北京基因型的成功克隆B0 / W148：用于快速检测和全局筛选的多重PCR分析
7. Rapid and Simultaneous Detection of Mycobacterium tuberculosis Complex and Beijing/W Genotype in Sputum by an Optimized DNA Extraction Protocol and a Novel Multiplex Real-Time PCR ▿ [O] . Leung, Eric T. Y., Zheng, L., Wong, Rity Y. K., 2011

机译：优化的DNA提取方案和新型实时荧光定量PCR快速同时检测痰中的结核分枝杆菌复合体和Beijing / W基因型

Russian “Successful” Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening

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