...
首页> 外文期刊>Journal of Clinical Microbiology >Comparative Evaluation of Three Commercial Quantitative Cytomegalovirus Standards by Use of Digital and Real-Time PCR
【24h】

Comparative Evaluation of Three Commercial Quantitative Cytomegalovirus Standards by Use of Digital and Real-Time PCR

机译:通过数字和实时PCR比较三种商业定量巨细胞病毒标准液的比较评估

获取原文
           

摘要

The recent development of the 1st WHO International Standard for human cytomegalovirus (CMV) and the introduction of commercially produced secondary standards have raised hopes of improved agreement among laboratories performing quantitative PCR for CMV. However, data to evaluate the trueness and uniformity of secondary standards and the consistency of results achieved when these materials are run on various assays are lacking. Three concentrations of each of the three commercially prepared secondary CMV standards were tested in quadruplicate by three real-time and two digital PCR methods. The mean results were compared in a pairwise fashion with nominal values provided by each manufacturer. The agreement of results among all methods for each sample and for like concentrations of each standard was also assessed. The relationship between the nominal values of standards and the measured values varied, depending upon the assay used and the manufacturer of the standards, with the degree of bias ranging from +0.6 to ?1.0 log10 IU/ml. The mean digital PCR result differed significantly among the secondary standards, as did the results of the real-time PCRs, particularly when plotted against nominal log10 IU values. Commercially available quantitative secondary CMV standards produce variable results when tested by different real-time and digital PCR assays, with various magnitudes of bias compared to nominal values. These findings suggest that the use of such materials may not achieve the intended uniformity among laboratories measuring CMV viral load, as envisioned by adaptation of the WHO standard.
机译:世卫组织第一个人类巨细胞病毒国际标准(CMV)的最新发展以及商业化生产的二级标准的引入,提高了进行CMV定量PCR的实验室之间达成更好协议的希望。但是,缺乏评估次要标准品的真实性和均一性以及这些材料在各种测定中运行时所获得结果的一致性的数据。通过三种实时和两种数字PCR方法,一式四份测试了三种商业制备的二级CMV标准溶液的三种浓度。将平均结果与每个制造商提供的标称值成对比较。还评估了每种方法和每种标准品的相似浓度在所有方法之间的结果一致性。标准的标称值与测量值之间的关系因所使用的测定方法和标准的制造商而异,偏差度范围为+0.6至?1.0 log 10 IU / ml 。次要标准品中,数字PCR的平均结果与实时PCR的结果之间存在显着差异,尤其是针对标称log 10 IU值绘制时。当通过不同的实时和数字PCR分析进行测试时,市售的定量二级CMV标准品会产生可变的结果,与标称值相比存在各种偏差。这些发现表明,按照世界卫生组织标准的修改,使用这种材料可能无法在测量CMV病毒载量的实验室之间达到预期的一致性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号