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首页> 外文期刊>Journal of Clinical Microbiology >Comparing Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Phenotypic and Molecular Methods for Identification of Species within the Streptococcus anginosus Group
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Comparing Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Phenotypic and Molecular Methods for Identification of Species within the Streptococcus anginosus Group

机译：比较基质辅助激光解吸电离-飞行时间质谱和表型和分子方法来鉴定链球菌性心绞痛组内的物种

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摘要

The heterogeneity of members of the Streptococcus anginosus group (SAG) has traditionally hampered their correct identification. Recently, the group was subdivided into 6 taxa whose prevalence among human infections is poorly described. We evaluated the accuracy of the Rapid ID32 Strep test, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), and a PCR multiplex method to identify 212 SAG isolates recovered from human infections to the species and subspecies level by using multilocus sequence analysis (MLSA) as the gold standard. We also determined the antimicrobial susceptibilities of the isolates. Representatives of all SAG taxa were found among our collection. MALDI-TOF MS and the Rapid ID32 Strep test correctly identified 92% and 68% of the isolates to the species level, respectively, but showed poor performance at the subspecies level, and the latter was responsible for major identification errors. The multiplex PCR method results were in complete agreement with the MLSA identifications but failed to distinguish the subspecies Streptococcus constellatus subsp. pharyngis and S. constellatus subsp. viborgensis. A total of 145 MLSA sequence types were present in our collection, indicating that within each taxon a number of different lineages are capable of causing infection. Significant antibiotic resistance was observed only to tetracycline, erythromycin, and clindamycin and was present in most taxa. MALDI-TOF MS is a reliable method for routine SAG species identification, while the need for identification to the subspecies level is not clearly established.
机译：传统上，心绞痛链球菌组（SAG）成员的异质性妨碍了他们的正确识别。最近，该组被细分为6个分类单元，其在人类感染中的流行率描述不高。我们评估了快速ID32 Strep测试，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）以及PCR多重检测方法的准确性，以鉴定从人类感染中恢复到物种和亚种水平的212种SAG分离物通过使用多基因座序列分析（MLSA）作为金标准。我们还确定了分离物的抗药性。在我们的收藏中发现了所有SAG分类单元的代表。 MALDI-TOF MS和Rapid ID32 Strep测试分别正确鉴定出了92％和68％的分离株，但在亚种水平上却表现不佳，而后者是造成重大鉴定错误的原因。多重PCR方法的结果与MLSA鉴定完全一致，但未能区分星座链球菌亚种。咽和星座链球菌亚种。维堡山我们的收藏集中共有145种MLSA序列类型，这表明在每个分类单元中，许多不同的谱系都能够引起感染。仅对四环素，红霉素和克林霉素观察到显着的抗生素抗性，并且在大多数分类单元中都存在。 MALDI-TOF MS是常规SAG物种鉴定的可靠方法，但对亚种水平鉴定的需求尚未明确。

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