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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of Metarhizium viride Mycosis in Veiled Chameleons (Chamaeleo calyptratus), Panther Chameleons (Furcifer pardalis), and Inland Bearded Dragons (Pogona vitticeps)
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Characterization of Metarhizium viride Mycosis in Veiled Chameleons (Chamaeleo calyptratus), Panther Chameleons (Furcifer pardalis), and Inland Bearded Dragons (Pogona vitticeps)

机译：在面纱变色龙（Chamaeleo calyptratus），黑豹变色龙（Furcifer pardalis）和内陆有胡子龙（Pogona vitticeps）中变绿变菌霉菌病的特征

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Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with clinical signs and pathological findings as well as improvement of diagnostic procedures. Beside 18S ribosomal DNA (rDNA) (small subunit [SSU]) and internal transcribed spacer region 1 (ITS-1), a fragment of the large subunit (LSU) of 28S rDNA, including domain 1 (D1) and D2, was sequenced for the identification of the fungus and phylogenetic analysis. Cultural isolation and histopathological examinations as well as the pattern of antifungal drug resistance, determined by using agar diffusion testing, were additionally used for comparison of the isolates. In total, 20 isolates from eight inland bearded dragons (Pogona vitticeps), six veiled chameleons (Chamaeleo calyptratus), and six panther chameleons (Furcifer pardalis) were examined. Most of the lizards suffered from fungal glossitis, stomatitis, and pharyngitis or died due to visceral mycosis. Treatment with different antifungal drugs according to resistance patterns in all three different lizard species was unsuccessful. Sequence analysis resulted in four different genotypes of M. viride based on differences in the LSU fragment, whereas the SSU and ITS-1 were identical in all isolates. Sequence analysis of the SSU fragment revealed the first presentation of a valid large fragment of the SSU of M. viride. According to statistical analysis, genotypes did not correlate with differences in pathogenicity, antifungal susceptibility, or species specificity.
机译：变态金属灭蚁菌已与变色龙中的致命性全身性霉菌病相关，但缺乏有关此真菌在爬行动物中引起的霉菌病的后续数据。因此，本研究的目的是获得有关圈养作为宠物饲养的爬行动物中的绿头孢霉菌的信息，以及其与临床体征和病理结果的关系以及诊断程序的改进。除了18S核糖体DNA（rDNA）（小亚基[SSU]）和内部转录间隔区1（ITS-1），还对28S rDNA大亚基（LSU）的片段进行了测序，包括结构域1（D1）和D2。用于真菌的鉴定和系统发育分析。通过琼脂扩散测试确定的文化分离和组织病理学检查以及抗真菌药物耐药性的模式还用于比较分离物。总共检查了来自八个内陆胡子龙（Pogona vitticeps），六个面纱变色龙（Chamaeleo calyptratus）和六个黑豹变色龙（Furcifer pardalis）的20个分离株。大多数蜥蜴患有真菌性舌炎，口腔炎和咽炎或因内脏真菌病而死亡。根据所有三种不同蜥蜴物种的耐药模式，使用不同的抗真菌药物进行治疗均未成功。序列分析基于LSU片段的差异产生了四种M. viride基因型，而SSU和ITS-1在所有分离物中均相同。 SSU片段的序列分析揭示了有效的大的M. viride SSU大片段。根据统计分析，基因型与致病性，抗真菌药性或物种特异性的差异不相关。

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