Misidentification of Mycobacterium paraense as Mycobacterium avium Complex by Accuprobe

Husain Poonawala; Vincent E. Piscitelli; Lynn Ladutko; Sheldon Campbell

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Misidentification of Mycobacterium paraense as Mycobacterium avium Complex by Accuprobe

机译：Accuprobe将副分枝杆菌错误鉴定为鸟分枝杆菌复合体

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A mycobacterial isolate from Veterans Affairs Hospital in Lake City, FL, was sent to the mycobacteriology reference laboratory at the West Haven Veterans Affairs Hospital (West Haven, CT) for further identification. Initial testing with the MAC probe was positive, with an RLU of 177,803. Subculture on solid media resulted in the growth of a scotochromogen with smooth yellow colonies. Small beaded acid-fast bacilli were seen on Kinyoun staining. Testing of the isolate using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) (Bruker Daltonics) suggested M. gordonae, but the score was below the cutoff of 1.7 necessary for reliable species identification.

机译：来自佛罗里达州莱克城的退伍军人事务医院的分枝杆菌分离株被送到西黑文退伍军人事务医院（康涅狄格州西黑文）的分枝杆菌学参考实验室进行进一步鉴定。 MAC探针的初始测试为阳性，RLU为177,803。在固体培养基上的继代培养导致了具有平滑黄色菌落的scotochromogen的生长。在Kinyoun染色中观察到小串珠的耐酸杆菌。使用基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF MS）（Bruker Daltonics）进行的分离株测试显示了戈登菌，但该分数低于进行可靠的物种鉴定所必需的1.7的临界值。

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《Journal of Clinical Microbiology》 |2017年第7期|共2页
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Husain Poonawala; Vincent E. Piscitelli; Lynn Ladutko; Sheldon Campbell;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii, and Mycobacterium gordonae directly from BACTEC TB broth cultures. [J] . B S Reisner, A M Gatson, G L Woods Journal of Clinical Microbiology . 1994,第12期

机译：使用Gen-Probe AccuProbes直接从BACTEC TB肉汤培养物中鉴定鸟分枝杆菌复合物，结核分枝杆菌复合物，堪萨斯分枝杆菌和戈登分枝杆菌。
2. Direct Identification of Mycobacterium avium Complex and Mycobacterium gordonae from MB/BacT Bottles Using AccuProbe [J] . Ana Paula S. Louro, Ken B. Waites, Ecaterina Georgescu, Journal of Clinical Microbiology . 2001,第2期

机译：使用AccuProbe从MB / BacT瓶中直接鉴定鸟分枝杆菌复合物和戈登分枝杆菌
3. Interpretive Criteria for Use of AccuProbe for Identification of Mycobacterium avium Complex Directly from 7H9 Broth Cultures [J] . Joann L. Cloud, Karen C. Carroll, Samuel Cohen, Journal of Clinical Microbiology . 2005,第7期

机译：AccuProbe用于直接从7H9肉汤培养物中鉴定鸟分枝杆菌复合物的解释性标准
4. Lipidomics of Mycobacterium avium subsp. paratuberculosis in comparison to other members of the M. avium complex [C] . Eckstein TM, Duskin SL, Chandrasekaran S, International Colloquium on Paratuberculosis . 2007

机译：分枝杆菌的脂多元族。与M. Avium Complex的其他成员相比，对抗麻痹
5. Molecular Genetic Analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium smegmatis [D] . Rathnaiah, Govardhan 2017

机译：鸟分枝杆菌亚种的分子遗传分析。副结核病和耻垢分枝杆菌
6. Misidentification of Mycobacterium paraense as Mycobacterium avium Complex by Accuprobe [O] . Husain Poonawala, Vincent E. Piscitelli, Lynn Ladutko, 2017

机译：Accuprobe将副分枝杆菌错误鉴定为鸟分枝杆菌复合体
7. Misidentification of Mycobacterium paraense as Mycobacterium avium Complex by Accuprobe [O] . Husain Poonawala, Vincent E. Piscitelli, Lynn Ladutko, 2017

机译：Accuprobe的分枝杆菌分枝杆菌的误诊

Misidentification of Mycobacterium paraense as Mycobacterium avium Complex by Accuprobe

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