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首页> 外文期刊>Journal of Clinical Microbiology >Toward Molecular Parasitologic Diagnosis: Enhanced Diagnostic Sensitivity for Filarial Infections in Mobile Populations
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Toward Molecular Parasitologic Diagnosis: Enhanced Diagnostic Sensitivity for Filarial Infections in Mobile Populations

机译：迈向分子寄生虫学诊断：流动人群丝虫感染的增强诊断敏感性。

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The diagnosis of filarial infections among individuals residing in areas where the disease is not endemic requires both strong clinical suspicion and expert training in infrequently practiced parasitological methods. Recently developed filarial molecular diagnostic assays are highly sensitive and specific but have limited availability and have not been closely evaluated for clinical use outside populations residing in areas of endemicity. In this study, we assessed the performance of a panel of real-time PCR assays for the four most common human filarial pathogens among blood and tissue samples collected from a cohort of patients undergoing evaluation for suspected filarial infections. Compared to blood filtration, real-time PCR was equally sensitive for the detection of microfilaremia due to Wuchereria bancrofti (2 of 46 samples positive by both blood filtration and PCR with no discordant results) and Loa loa (24 of 208 samples positive by both blood filtration and PCR, 4 samples positive by PCR only, and 3 samples positive by blood filtration only). Real-time PCR of skin snip samples was significantly more sensitive than microscopic examination for the detection of Onchocerca volvulus microfiladermia (2 of 218 samples positive by both microscopy and PCR and 12 samples positive by PCR only). The molecular assays required smaller amounts of blood and tissue than conventional methods and could be performed by laboratory personnel without specialized parasitology training. Taken together, these data demonstrate the utility of the molecular diagnosis of filarial infections in mobile populations.
机译：在非疾病流行地区居住的人中，要进行丝虫感染的诊断既需要强有力的临床怀疑，也需要接受不常用的寄生虫学方法的专家培训。最近开发的丝状分子诊断测定法具有很高的敏感性和特异性，但可用性有限，尚未针对在流行地区居住的人群进行临床评估。在这项研究中，我们评估了一组实时PCR检测方法的性能，该方法针对从正在评估可疑丝虫感染的患者队列中收集的血液和组织样本中的四种最常见的人丝虫病原体。与血液滤过相比，实时荧光定量PCR对由 Wuchereria bancrofti 引起的微丝虫病检测同样敏感（血液滤过和PCR均为阳性的46个样品中有2个没有差异）结果）和 L oa loa （血液过滤和PCR均为阳性的208个样本中有24个，仅PCR呈阳性的4个样本中，有3个样本仅通过血液过滤呈阳性）。皮肤片段实时荧光定量PCR检测细小On皮的敏感性远高于显微镜检查（显微镜和PCR检测均为阳性的218个样品中有2个和12个样品）仅通过PCR检测为阳性）。与常规方法相比，分子分析所需的血液和组织量更少，并且可以由实验室人员进行，而无需经过专门的寄生虫学培训。综上所述，这些数据证明了流动人群中丝虫感染分子诊断的实用性。

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