Efficient Quantification of HIV-1 in Heparin Plasma Spiked with Cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 Tests

Linda L. Jagodzinski; Holly R. Weston; Ying Liu; Robert J. OConnell; Sheila A. Peel

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Efficient Quantification of HIV-1 in Heparin Plasma Spiked with Cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 Tests

机译：通过Roche Cobas TaqMan和Abbott RealTime HIV-1测试对培养的HIV-1掺入的肝素血浆中的HIV-1进行高效定量

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The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott RealTime test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.

机译：FDA批准了当前的自动实时HIV-1病毒载量测定法，Roche Cobas AmpliPrep / Cobas TaqMan测试和Abbott RealTime测试，已与EDTA血浆一起使用。我们显示，这两个实时逆转录PCR（RT-PCR）测试可靠地量化了掺有HIV-1分离株MN的肝素血浆标本中的HIV-1 RNA。

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《Journal of Clinical Microbiology》 |2012年第8期|共3页
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Linda L. Jagodzinski; Holly R. Weston; Ying Liu; Robert J. OConnell; Sheila A. Peel;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Ability of Two Commercially Available Assays (Abbott RealTime HIV-1 and Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 Version 2.0) To Quantify Low HIV-1 RNA Levels (<1,000 Copies/Milliliter): Comparison with Clinical Samples and NIBSC Working Reagent for Nucleic Acid Testing Assays [J] . Alessandra Amendola, Patrizia Marsella, Maria Bloisi, Journal of Clinical Microbiology . 2014,第6期

机译：两种商业化检测方法（Abbott RealTime HIV-1和Roche Cobas AmpliPrep / Cobas TaqMan HIV-1 2.0版）对低HIV-1 RNA水平（<1,000份/毫升）进行定量的能力：与临床样品和NIBSC工作试剂的比较核酸检测方法
2. High correlation between the Roche COBAS ? AmpliPrep/COBAS ? TaqMan ? HIV-1, v2.0 and the Abbott m2000 RealTime HIV-1 assays for quantification of viral load in HIV-1 B and non-B subtypes [J] . Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2011,第3期

机译：罗氏COBAS之间的相关性高吗？ AmpliPrep / COBAS？ TaqMan？ HIV-1，v2.0和Abbott m2000 RealTime HIV-1测定法可量化HIV-1 B和非B亚型的病毒载量
3. Comparison of the bioMerieux NucliSENS EasyQ HIV-1 v2.0-HIV-1 RNA quantification assay versus Abbott RealTime HIV-1 and Roche Cobas TaqMan HIV-1 v2.0 on current epidemic HIV-1 variants [J] . Mourez Thomas, Delaugerre Constance, Vray Muriel, Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2015,第Null期

机译：比较BioMerieux NucliSENS EasyQ HIV-1 v2.0-HIV-1 RNA定量测定法与雅培RealTime HIV-1和Roche Cobas TaqMan HIV-1 v2.0在当前流行的HIV-1变体上的比较
4. Development of Ultrasensitive Nanoscale LC/MS Techniques for Quantification of an HIV-1 Integrase Inhibitor in Human Plasma [C] . Miaoqing Shen, Li Sun, Kevin Bateman, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：超敏纳米级LC / MS技术的发展，用于定量人血浆中HIV-1整合酶抑制剂的定量
5. Characterizing the Expression and Localization of Drug Transporters and Metabolic Enzymes in the testes of Uninfected and HIV-1 Infected, Treated Subjects - Potential Contribution to an HIV-1 Sanctuary. [D] . Huang, Yiying Billy. 2015

机译：表征在未感染和HIV-1感染，治疗的受试者的睾丸中药物转运蛋白和代谢酶的表达和定位-对HIV-1避难所的潜在贡献。
6. Efficient Quantification of HIV-1 in Heparin Plasma Spiked with Cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 Tests [O] . Linda L. Jagodzinski, Holly R. Weston, Ying Liu, 2012

机译：通过Roche Cobas TaqMan和Abbott RealTime HIV-1测试对培养的HIV-1掺入的肝素血浆中的HIV-1进行高效定量
7. Ability of Two Commercially Available Assays (Abbott RealTime HIV-1 and Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 Version 2.0) To Quantify Low HIV-1 RNA Levels (<1,000 Copies/Milliliter): Comparison with Clinical Samples and NIBSC Working Reagent for Nucleic Acid Testing Assays [O] . A. Amendola, P. Marsella, M. Bloisi, 2014

机译：两种市售的测定的能力（Abbott Realtime HIV-1和Roche Cobas Ampliprep / Cobas Taqman HIV-1版本2.0），以定量低HIV-1 RNA水平（<1,000拷贝/毫升）：与临床样品和NIBSC工作试剂的比较核酸测试测定

Efficient Quantification of HIV-1 in Heparin Plasma Spiked with Cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 Tests

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