Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

Choon-Mee Kim; Min Keun Cho; Dong-Min Kim; Na-Ra Yun; Seok Won Kim; Sook Jin Jang

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Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

机译：Ot-16sRF1和Ot-16sRR1引物靶向16S rRNA基因的常规PCR的诊断斑疹伤寒的准确性：病例对照研究。

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We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.

机译：我们回顾性地使用Ot-16sRF1 / Ot-16sRR1引物诊断灌木斑疹伤寒，以16S rRNA基因为靶标的常规PCR的准确性（16S C-PCR）。与其他靶标相比，通过16S C-PCR诊断虫东方型感染的敏感性提高了87.0％，特异性提高了100％，因此是一种简单且临床有用的方法，具有良好的诊断准确性。

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《Journal of Clinical Microbiology》 |2016年第1期|共2页
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Choon-Mee Kim; Min Keun Cho; Dong-Min Kim; Na-Ra Yun; Seok Won Kim; Sook Jin Jang;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

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